A quantitative imaging-based screen reveals the exocyst as a network hub connecting endocytosis and exocytosis
| العنوان: | A quantitative imaging-based screen reveals the exocyst as a network hub connecting endocytosis and exocytosis |
|---|---|
| المؤلفون: | Jean-Baptiste Sibarita, Anne Royou, Romain Mitteau, Christophe Velours, Aurelie Massoni-Laporte, Mini Jose, Sylvain Tollis, Deepak T. Nair, Derek McCusker |
| المساهمون: | Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Centre de physique moléculaire optique et hertzienne (CPMOH), Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1, Interdisciplinary Institute for Neuroscience (IINS), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), University of California [Santa Cruz] (UCSC), University of California, Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS), University of California [Santa Cruz] (UC Santa Cruz), University of California (UC) |
| المصدر: | Molecular Biology of the Cell Molecular Biology of the Cell, American Society for Cell Biology, 2015, 26 (13), pp.2519-2534. ⟨10.1091/mbc.E14-11-1527⟩ Molecular Biology of the Cell, 2015, 26 (13), pp.2519-2534. ⟨10.1091/mbc.E14-11-1527⟩ |
| بيانات النشر: | American Society for Cell Biology (ASCB), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Saccharomyces cerevisiae Proteins, Polarity (physics), [SDV]Life Sciences [q-bio], Cellular polarity, Endocytic cycle, Exocyst, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, Endocytosis, Exocytosis, 03 medical and health sciences, 0302 clinical medicine, Cell polarity, Molecular Biology, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, Cell Polarity, Articles, Cell Biology, Transport protein, Cell biology, Protein Transport, Membrane Trafficking, Saccharomycetales, Metabolic Networks and Pathways, 030217 neurology & neurosurgery |
| الوصف: | The mechanisms governing the spatial organization of endocytosis and exocytosis are ill defined. A quantitative imaging screen and high-density single-vesicle tracking are used to identify mutants that are defective in endocytic and exocytic vesicle organization. The screen identifies a role for the exocyst complex in connecting the two pathways. The coupling of endocytosis and exocytosis underlies fundamental biological processes ranging from fertilization to neuronal activity and cellular polarity. However, the mechanisms governing the spatial organization of endocytosis and exocytosis require clarification. Using a quantitative imaging-based screen in budding yeast, we identified 89 mutants displaying defects in the localization of either one or both pathways. High-resolution single-vesicle tracking revealed that the endocytic and exocytic mutants she4∆ and bud6∆ alter post-Golgi vesicle dynamics in opposite ways. The endocytic and exocytic pathways display strong interdependence during polarity establishment while being more independent during polarity maintenance. Systems analysis identified the exocyst complex as a key network hub, rich in genetic interactions with endocytic and exocytic components. Exocyst mutants displayed altered endocytic and post-Golgi vesicle dynamics and interspersed endocytic and exocytic domains compared with control cells. These data are consistent with an important role for the exocyst in coordinating endocytosis and exocytosis. |
| تدمد: | 1939-4586 1059-1524 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83cdb6421af9fb5eabb50b4beab89ede https://doi.org/10.1091/mbc.e14-11-1527 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....83cdb6421af9fb5eabb50b4beab89ede |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
|---|