miR‐30b protects nigrostriatal dopaminergic neurons from MPP(+)‐induced neurotoxicity via SNCA
| العنوان: | miR‐30b protects nigrostriatal dopaminergic neurons from MPP(+)‐induced neurotoxicity via SNCA |
|---|---|
| المؤلفون: | Zhuo‐ying Zhu, Yanping Wang, Shuxia Qian, Yufei Shen, Congying Xu |
| المصدر: | Brain and Behavior, Vol 10, Iss 4, Pp n/a-n/a (2020) Brain and Behavior |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 1-Methyl-4-phenylpyridinium, Cell Survival, Parkinson's disease, 050105 experimental psychology, lcsh:RC321-571, Pathogenesis, 03 medical and health sciences, Behavioral Neuroscience, 0302 clinical medicine, Downregulation and upregulation, Western blot, Parkinsonian Disorders, Cell Line, Tumor, medicine, Humans, 0501 psychology and cognitive sciences, Luciferase, Viability assay, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, Original Research, medicine.diagnostic_test, Chemistry, Dopaminergic Neurons, 05 social sciences, Dopaminergic, Neurotoxicity, apoptosis, medicine.disease, Cell biology, Up-Regulation, MicroRNAs, HEK293 Cells, Proto-Oncogene Proteins c-bcl-2, neurotoxicology, Apoptosis, alpha-Synuclein, 030217 neurology & neurosurgery, miR‐30b |
| الوصف: | Objective To explore the function of miR‐30b in pathogenesis of Parkinson's disease (PD) and its underlying molecular mechanism. Materials and Methods We used 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPP(+)) as a tool for constructing the PD cell model, using miR‐30b mimics or inhibitors to manipulate miR‐30b level for an experimental model of acquisition. The cell viability of SH‐SY5Y was detected by CCK, and luciferase was used to screen the binding of target genes. The protein levels of SNCA were measured by Western blot. Then, we investigate the changes in pro‐ and anti‐apoptotic markers with or without miR‐30b treatment. Results There was a significant low expression of MiR‐30b in MPP(+)‐induced cells. SH‐SY5Y cell viability was rescued by MiR‐30b overexpression. Luciferase experiments showed that MiR‐30b may bind to the 3′‐UTR side of SNCA and inhibited its expression. By Western blot, the SNCA level was markedly decreased by miR‐30b. miR‐30b attenuated the upregulation of Bax and the depletion of Bcl‐2 induced by MPP(+). miR‐30b SNCA Parkinson's disease. |
| اللغة: | English |
| تدمد: | 2162-3279 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::841edda0d78ffcf5ac865e3f1c010edd https://doaj.org/article/27d64e3449ed4ed8a7d0011e442f6b5d |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....841edda0d78ffcf5ac865e3f1c010edd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21623279 |
|---|