The RFG oligomerization domain mediates kinase activation and re-localization of the RET/PTC3 oncoprotein to the plasma membrane
| العنوان: | The RFG oligomerization domain mediates kinase activation and re-localization of the RET/PTC3 oncoprotein to the plasma membrane |
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| المؤلفون: | Alfredo Fusco, Giovanna Maria Pierantoni, Claudia De Lorenzo, Maria Terasa Berlingieri, Carmen Monaco, Giancarlo Vecchio, Roberta Visconti, Maria Vittoria Barone, A. Mineo, Massimo Santoro |
| المساهمون: | C., Monaco, Visconti, R., Barone, MARIA VITTORIA, Pierantoni, GIOVANNA MARIA, Berlingieri, M. T., DE LORENZO, Claudia, Mineo, A., Vecchio, Giancarlo, Fusco, Alfredo, Santoro, Massimo |
| المصدر: | Oncogene (Basingstoke) 20 (2001): 599–608. info:cnr-pdr/source/autori:Monaco C; Visconti R; Barone MV; Pierantoni GM; Berlingieri MT; De Lorenzo C; Mineo A; Vecchio G; Fusco A; Santoro M/titolo:The RFG oligomerization domain mediates kinase activation and re-localization of the RET%2FPTC3 oncoprotein to the plasma membrane/doi:/rivista:Oncogene (Basingstoke)/anno:2001/pagina_da:599/pagina_a:608/intervallo_pagine:599–608/volume:20 |
| بيانات النشر: | Macmillan, Basingstoke , Regno Unito, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Cancer Research, congenital, hereditary, and neonatal diseases and abnormalities, endocrine system, Oncogene Proteins, Oncogene Proteins, Fusion, endocrine system diseases, Thyroid Gland, Biology, Transfection, 3T3 cells, thyroid, Cell Line, Enzyme activator, Mice, Protein structure, Proto-Oncogene Proteins, Genetics, medicine, Animals, Drosophila Proteins, Humans, Molecular Biology, neoplasms, dimerization, coiled coil, Kinase, transformation, Cell Membrane, Proto-Oncogene Proteins c-ret, tyrosine kinase, Receptor Protein-Tyrosine Kinases, Epithelial Cells, 3T3 Cells, Protein-Tyrosine Kinases, Cell biology, Protein Structure, Tertiary, Rats, Enzyme Activation, medicine.anatomical_structure, Cell Transformation, Neoplastic, Biochemistry, Tyrosine kinase |
| الوصف: | The RET/PTC3 oncogene arises from the fusion between the N-terminal encoding domain of the RFG gene and the tyrosine kinase encoding domain of RET receptor. RET/PTC3 is very frequent in papillary thyroid carcinomas, especially in children exposed to the Chernobyl accident. We have studied the functional consequences of the RFG-RET fusion. Here we show that the N-terminal coiled-coil domain of RGF mediates oligomerization and activation of the kinase and of the transforming capability of RET/PTC3. In addition, the RFG coiled-coil domain mediates a physical association between RET/PTC3 and RGF proteins, rendering RFG a bona fide substrate of RET/PTC3 kinase. Finally, we show that the coiled-coil domain of RGF is essential for the distribution of the RET/PTC3 protein at the membrane/particulate cell compartment level, where also most of the RFG protein is localized. We propose that fusion to the RFG coiled-coil domain provides RET kinase with a scaffold that mediates oligomerization and re-localization of the RET/PTC3 protein, a process that may be crucial for the signalling of this specific RET/PTC variant. |
| وصف الملف: | STAMPA |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::842fd2dd31dd02dd85bc3eed4de48407 http://www.cnr.it/prodotto/i/20855 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....842fd2dd31dd02dd85bc3eed4de48407 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |