Matrix Effects on the Microcystin-LR Fluorescent Immunoassay Based on Optical Biosensor
| العنوان: | Matrix Effects on the Microcystin-LR Fluorescent Immunoassay Based on Optical Biosensor |
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| المؤلفون: | Miao He, Hanchang Shi, Anna Zhu, Jianwu Sheng, Feng Long |
| المصدر: | Sensors, Vol 9, Iss 4, Pp 3000-3010 (2009) Sensors (Basel, Switzerland) Sensors; Volume 9; Issue 4; Pages: 3000-3010 |
| بيانات النشر: | MDPI AG, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Matrix effects, Analytical chemistry, Microcystin-LR, lcsh:Chemical technology, Biochemistry, Article, Analytical Chemistry, chemistry.chemical_compound, medicine, Humic acid, Chelation, lcsh:TP1-1185, Electrical and Electronic Engineering, fluorescent immunoassay, optical biosensor, Instrumentation, IC50, chemistry.chemical_classification, Chromatography, medicine.diagnostic_test, Fluorescence, Atomic and Molecular Physics, and Optics, Working range, chemistry, Immunoassay, Reagent |
| الوصف: | Matrix effects on the microcystin-LR fluorescent immunoassay based on the evanescent wave all-fiber immunosensor (EWAI) and their elimination methods were studied. The results indicated that PBS and humic acid did not affect the monitoring of samples under the investigated conditions. When the pH was less than 6 or higher than 8, the fluorescence signals detected by immunosensor systems were obviously reduced with the decrease or increase of pH. When the pH ranged from 6 to 8, IC(50) and the linear working range of MC-LR calculated from the detection curves were 1.01∼1.04 μg/L and 0.12∼10.5 μg/L, respectively, which was favourable for an MC-LR immunoassay. Low concentrations of Cu(2+) rarely affected the detection performance of MC-LR. When the concentration of CuSO(4) was higher than 5 mg/L, the fluorescence signal detected by EWAI clearly decreased, and when the concentration of CuSO(4) was 10 mg/L, the fluorescence signal detected was reduced by 70%. The influence of Cu(2+) on the immunoassay could effectively be compromised when chelating reagent EDTA was added to the pre-reaction mixture. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1424-8220 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8431b3b17557389fcc1b8514da5c41a5 http://www.mdpi.com/1424-8220/9/4/3000/ |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8431b3b17557389fcc1b8514da5c41a5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14248220 |
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