Detection of Human CD38 Using Variable Lymphocyte Receptor (VLR) Tetramers
| العنوان: | Detection of Human CD38 Using Variable Lymphocyte Receptor (VLR) Tetramers |
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| المؤلفون: | Nikolaus E. Wolter, Evan J. Propst, Mario A. Ostrowski, Yanling Liu, Eyal Grunebaum, Laura M. Ernst, Patrick Budylowski, Srijit Khan, Paolo Campisi, Leslie Y. T. Leung, Shilan Dong, Götz R. A. Ehrhardt |
| المصدر: | Cells, Vol 9, Iss 950, p 950 (2020) Cells |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Models, Molecular, CD38, Flow cytometry, Variable lymphocyte receptor, Antigen, immune system diseases, Cell surface receptor, evolution, medicine, Centroblasts, Humans, Lymphocytes, lcsh:QH301-705.5, biology, medicine.diagnostic_test, tetramer, Chemistry, Brief Report, flow cytometry, Germinal center, General Medicine, ADP-ribosyl Cyclase 1, Cell biology, lcsh:Biology (General), biology.protein, variable lymphocyte receptor (VLR), Antibody |
| الوصف: | CD38 is a multifunctional cell surface receptor expressed on multiple cell lineages of hematopoietic origin with high levels of expression on human plasma cells. Previously, we isolated the monoclonal variable lymphocyte receptor B (VLRB) MM3 antibody from the evolutionarily distant sea lamprey, which recognized the CD38 ectoenzyme exclusively on human plasma cells in a manner that correlated with CD38 enzymatic activity. The plasma cell-specific binding of VLRB MM3 contrasts with the broad pattern of expression of CD38-determined conventional antibodies specific for this antigen. In an effort to facilitate the application of this unique reagent in combination with conventional antibody panels, we explored a strategy to generate VLRB MM3 tetramers. The resulting reagent maintained the threshold-based recognition of CD38. Increased sensitivity achieved with VLRB MM3 tetramers also showed preferential recognition of germinal center centroblasts over centrocytes. VLRB MM3 tetramers thus provided a unique and versatile single-step staining reagent for the detection of human CD38 that is readily incorporated into multi-color flow cytometry panels. |
| تدمد: | 2073-4409 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84658ff8a6a8cb9852be1398f0d22570 https://doi.org/10.3390/cells9040950 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....84658ff8a6a8cb9852be1398f0d22570 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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