Inhibitory Effect of TNF-α on Malaria Pre-Erythrocytic Stage Development: Influence of Host Hepatocyte/Parasite Combinations
| العنوان: | Inhibitory Effect of TNF-α on Malaria Pre-Erythrocytic Stage Development: Influence of Host Hepatocyte/Parasite Combinations |
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| المؤلفون: | Jean-Marc Chavatte, Laurent Rénia, Jean Francois Franetich, Robert W. Sauerwein, Nadya Depinay, Marjorie Mauduit, Dominique Mazier, Laurent Hannoun, Adrian J. F. Luty, Anne Charlotte Grüner, Georges Snounou, Jean-Michel Siksik, Geert-Jan van Gemert |
| المصدر: | PLoS One, 6 PLoS One, 6, 3 PLoS ONE, Vol 6, Iss 3, p e17464 (2011) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Erythrocytes, medicine.medical_treatment, Protozoology, Mice, Molecular Cell Biology, Receptor, Multidisciplinary, Interleukin, Host-Pathogen Interaction, Infectious Diseases, medicine.anatomical_structure, Cytokine, Medical Microbiology, Hepatocyte, Cytokines, Medicine, Tumor necrosis factor alpha, Plasmodium yoelii, Research Article, Clinical Research Design, Preclinical Models, Science, Plasmodium falciparum, Immunology, Gastroenterology and Hepatology, Biology, Nitric Oxide, Microbiology, Host-Parasite Interactions, Tetraspanin 28, Antigens, CD, Cell Line, Tumor, Parasitic Diseases, medicine, Animals, Humans, Life Cycle Stages, Tumor Necrosis Factor-alpha, Immunity, Tropical Diseases (Non-Neglected), Immune Defense, Pathogenesis and modulation of inflammation Infection and autoimmunity [N4i 1], biology.organism_classification, medicine.disease, Malaria, Solubility, Immune System, Hepatocytes, Parastic Protozoans, Parasitology, Clinical Immunology, Reactive Oxygen Species |
| الوصف: | Contains fulltext : 97044.pdf (Publisher’s version ) (Open Access) BACKGROUND: The liver stages of malaria parasites are inhibited by cytokines such as interferon-gamma or Interleukin (IL)-6. Binding of these cytokines to their receptors at the surface of the infected hepatocytes leads to the production of nitric oxide (NO) and radical oxygen intermediates (ROI), which kill hepatic parasites. However, conflicting results were obtained with TNF-alpha possibly because of differences in the models used. We have reassessed the role of TNF-alpha in the different cellular systems used to study the Plasmodium pre-erythrocytic stages. METHODS AND FINDINGS: Human or mouse TNF-alpha were tested against human and rodent malaria parasites grown in vitro in human or rodent primary hepatocytes, or in hepatoma cell lines. Our data demonstrated that TNF-alpha treatment prevents the development of malaria pre-erythrocytic stages. This inhibitory effect however varies with the infecting parasite species and with the nature and origin of the cytokine and hepatocytes. Inhibition was only observed for all parasite species tested when hepatocytes were pre-incubated 24 or 48 hrs before infection and activity was directed only against early hepatic parasite. We further showed that TNF-alpha inhibition was mediated by a soluble factor present in the supernatant of TNF-alpha stimulated hepatocytes but it was not related to NO or ROI. Treatment TNF-alpha prevents the development of human and rodent malaria pre-erythrocytic stages through the activity of a mediator that remains to be identified. CONCLUSIONS: Treatment TNF-alpha prevents the development of human and rodent malaria pre-erythrocytic stages through the activity of a mediator that remains to be identified. However, the nature of the cytokine-host cell-parasite combination must be carefully considered for extrapolation to the human infection. |
| وصف الملف: | application/pdf |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::849649bcb4d0d1a15e68e0db98547d18 https://doi.org/10.1371/journal.pone.0017464 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....849649bcb4d0d1a15e68e0db98547d18 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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