Upregulation of cannabinoid receptor type 2, but not TSPO, in senescence-accelerated neuroinflammation in mice: a positron emission tomography study
العنوان: | Upregulation of cannabinoid receptor type 2, but not TSPO, in senescence-accelerated neuroinflammation in mice: a positron emission tomography study |
---|---|
المؤلفون: | Hideo Tsukada, Masato Nakamura, Shingo Nishiyama, Satoru Yamagishi, Dai Fukumoto, Takeharu Kakiuchi, Yasuomi Ouchi, Hiroyuki Ohba, Kohji Sato, Chika Takizawa, Yurika Iga |
المصدر: | Journal of Neuroinflammation, Vol 16, Iss 1, Pp 1-10 (2019) Journal of Neuroinflammation |
بيانات النشر: | BMC, 2019. |
سنة النشر: | 2019 |
مصطلحات موضوعية: | 0301 basic medicine, Senescence, medicine.medical_specialty, Aging, Positron emission tomography, Senescence-accelerated prone mouse, Immunology, Immunostaining, Neuroprotection, lcsh:RC346-429, Receptor, Cannabinoid, CB2, 03 medical and health sciences, Cellular and Molecular Neuroscience, Mice, 0302 clinical medicine, Receptors, GABA, Internal medicine, medicine, Translocator protein, Cannabinoid receptor type 2, Animals, Microglial activation, Neuroinflammation, lcsh:Neurology. Diseases of the nervous system, Inflammation, Microglia, biology, business.industry, General Neuroscience, Research, Neurodegeneration, Brain, medicine.disease, Endocannabinoid system, Up-Regulation, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Neurology, Positron-Emission Tomography, biology.protein, lipids (amino acids, peptides, and proteins), Radiopharmaceuticals, business, 030217 neurology & neurosurgery |
الوصف: | Background Microglial cells are activated in response to changes in brain homeostasis during aging, dementia, and stroke. Type 2 endocannabinoid receptors (CB2) and translocator protein 18 kD (TSPO) are considered to reflect distinct aspects of microglia-related neuroinflammatory responses in the brain. CB2 activation is considered to relate to the neuroprotective responses that may occur predominantly in the early stage of brain disorders such as Alzheimer’s disease, while an increase in TSPO expression tends to occur later during neuroinflammation, in a proinflammatory fashion. However, this information was deduced from studies with different animal samples under different experimental settings. In this study, we aimed to examine the early microglial status in the inflammation occurring in the brains of senescence-accelerated mouse prone 10 (SAMP10) mice, using positron emission tomography (PET) with CB2 and TSPO tracers, together with immunohistochemistry. Methods Five- and 15-week-old SAMP10 mice that undergo neurodegeneration after 7 months of age were used. The binding levels of the TSPO tracer (R)-[11C]PK11195 and CB2 tracer [11C]NE40 were measured using PET in combination with immunohistochemistry for CB2 and TSPO. To our knowledge, this is the first study to report PET data for CB2 and TSPO at the early stage of cognitive impairment in an animal model. Results The standard uptake value ratios (SUVRs) of [11C]NE40 binding were significantly higher than those of (R)-[11C]PK11195 binding in the cerebral cortical region at 15 weeks of age. At 5 weeks of age, the [11C]NE40 SUVR tended to be higher than the (R)-[11C]PK11195 SUVR. The (R)-[11C]PK11195 SUVR did not significantly differ between 5- and 15-week-old mice. Consistently, immunostaining analysis confirmed the upregulation of CB2, but not TSPO. Conclusions The use of the CB2 tracer [11C]NE40 and/or an immunohistochemical approach allows evaluation of the role of microglia in acute neuroinflammatory processes in the early stage of neurodegeneration. The present results provide in vivo evidence of different responses of two types of microglia to senescence-accelerated neuroinflammation, implying the perturbation of microglial balance by aging. Specific treatment for CB2-positive microglia might help ameliorate senescence-related neuroinflammation and the following neurodegeneration. |
اللغة: | English |
تدمد: | 1742-2094 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84974d4bb72982ddc315bc0bf4364cb2 http://link.springer.com/article/10.1186/s12974-019-1604-3 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....84974d4bb72982ddc315bc0bf4364cb2 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 17422094 |
---|