Induction of plant resistance is a tool that can be incorporated into the integrated management of diseases of cultivated plants. In this way, this work aims to evaluate the elicitor action of biocontrol agents in the induction of glyceolin. In this study were used concentrations about 0; 0.5; 1; 1.5 and 2% diluted in water; spore suspension of Trichoderma asperellum BV10, Bacillus subtilis BV02 and Bacillus amyloliquefaciens BV03 microorganisms. To determine the glyceolin, cotyledons were grown in sand, weighed and cut in longitudinal section on the lower surface. Subsequently, these were deposited 50 μL of the concentrations in the cuts and the extraction performed in H2O and concentration determined by absorbance at wavelength 285nm. The results were submitted to analysis of variance and compared by the regression test (p
