Plasma membrane associated transcription of cytoplasmic DNA
| العنوان: | Plasma membrane associated transcription of cytoplasmic DNA |
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| المؤلفون: | Teresa M. Jones, Richard A. Lerner, Ali Torkamani, Julong Cheng, Yingjie Peng |
| المصدر: | Proceedings of the National Academy of Sciences. 109:10827-10831 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Cytoplasm, Transcription, Genetic, Centromere, Molecular Sequence Data, RNA-dependent RNA polymerase, RNA polymerase II, DNA, Satellite, Chromosomes, Cell Line, Transcription (biology), RNA polymerase I, Humans, RNA, Messenger, RNA polymerase II holoenzyme, Polymerase, Transcription bubble, B-Lymphocytes, Multidisciplinary, Base Sequence, biology, Cell Membrane, Biological Sciences, Molecular biology, RNA, Ribosomal, biology.protein, RNA Polymerase II, Transcription factor II D |
| الوصف: | Cytoplasmic membrane-associated DNA (cmDNA) is a species of DNA that attaches to the plasma membrane and has physical and chemical properties that differ from those of bulk chromosomal and mitochondrial DNAs. Here, we used deep sequencing to analyze cmDNA and showed that satellite DNAs consisting of both of simple (CCATT) N repeats from the pericentromere regions of the chromosomes and 171-bp α-satellite repeat sequences from centromeres were highly enriched. Importantly, we found there is a special cytoplasmic membrane-associated transcription system in which DNA-dependent RNA polymerase II, which colocalizes with template cmDNA at the plasma membrane, can transcribe the membrane-associated 171-bp α-satellite repeat sequences into RNA. Analysis of phosphorylation patterns indicated that the RNA polymerase II in the plasma membrane is in a different chemical state from its nuclear counterpart. |
| تدمد: | 1091-6490 0027-8424 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84c8d11e9f1062befb861b85948108f5 https://doi.org/10.1073/pnas.1208716109 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....84c8d11e9f1062befb861b85948108f5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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