Spatially Mapping the Baseline and Bisphenol-A Exposed Daphnia magna Lipidome Using Desorption Electrospray Ionization—Mass Spectrometry
| العنوان: | Spatially Mapping the Baseline and Bisphenol-A Exposed Daphnia magna Lipidome Using Desorption Electrospray Ionization—Mass Spectrometry |
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| المؤلفون: | Ralf Weber, Matthew Smith, Mark Viant |
| المصدر: | Metabolites, Vol 12, Iss 33, p 33 (2022) Metabolites Metabolites; Volume 12; Issue 1; Pages: 33 |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Endocrinology, Diabetes and Metabolism, Daphnia magna, mass spectrometry imaging, Biochemistry, Microbiology, Article, QR1-502, desorption electrospray ionization, lipidome, ion images, bisphenol-A, egg development, cryosectioning, Molecular Biology |
| الوصف: | Untargeted lipidomics has previously been applied to the study of daphnids and the discovery of biomarkers that are indicative of toxicity. Typically, liquid chromatography—mass spectrometry is used to measure the changes in lipid abundance in whole-body homogenates of daphnids, each only ca. 3 mm in length which limits any biochemical interpretation of site-specific toxicity. Here, we applied mass spectrometry imaging of Daphnia magna to combine untargeted lipidomics with spatial resolution to map the molecular perturbations to defined anatomical regions. A desorption electrospray ionization—mass spectrometry (DESI-MS) method was optimized and applied to tissue sections of daphnids exposed to bisphenol-A (BPA) compared to unexposed controls, generating an untargeted mass spectrum at each pixel (35 µm2/pixel) within each section. First, unique lipid profiles from distinct tissue types were identified in whole-body daphnids using principal component analysis, specifically distinguishing appendages, eggs, eye, and gut. Second, changes in the lipidome were mapped over four stages of normal egg development and then the effect of BPA exposure on the egg lipidome was characterized. The primary perturbations to the lipidome were annotated as triacylglycerides and phosphatidylcholine, and the distributions of the individual lipid species within these classes were visualized in whole-body D. magna sections as ion images. Using an optimized DESI-MS workflow, the first ion images of D. magna tissue sections were generated, mapping both their baseline and BPA-perturbed lipidomes. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2218-1989 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84f67799583d8c02dfffba15c9f2ea70 https://www.mdpi.com/2218-1989/12/1/33 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....84f67799583d8c02dfffba15c9f2ea70 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22181989 |
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