Mouse Hippocampal Organotypic Tissue Cultures Exposed to In Vitro 'Ischemia' Show Selective and Delayed CA1 Damage that is Aggravated by Glucose
| العنوان: | Mouse Hippocampal Organotypic Tissue Cultures Exposed to In Vitro 'Ischemia' Show Selective and Delayed CA1 Damage that is Aggravated by Glucose |
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| المؤلفون: | Tobias Cronberg, Sailasree Nemali, Tadeusz Wieloch, Anna Rytter, Fredrik Asztely |
| المصدر: | Scopus-Elsevier |
| بيانات النشر: | SAGE Publications, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | medicine.medical_specialty, Ischemia, Hippocampus, Receptors, N-Methyl-D-Aspartate, Brain Ischemia, 030218 nuclear medicine & medical imaging, Brain ischemia, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, In vivo, Culture Techniques, Internal medicine, Extracellular fluid, medicine, Animals, Receptors, AMPA, Propidium iodide, Hypoxia, Cell damage, Cerebrospinal Fluid, Ions, Mice, Inbred BALB C, business.industry, Glutamate receptor, medicine.disease, Electrophysiology, Glucose, Endocrinology, Neurology, chemistry, Cell culture, Immunology, Potassium, Calcium, Neurology (clinical), Dizocilpine Maleate, Cardiology and Cardiovascular Medicine, business, Excitatory Amino Acid Antagonists, 030217 neurology & neurosurgery, Hydrogen |
| الوصف: | Oxygen and glucose deprivation (OGD) in cell cultures is generally studied in a medium, such as artificial cerebrospinal fluid (CSF), with an ion composition similar to that of the extracellular fluid of the normal brain (2 to 4 mmol/L K+, 2 to 3 mmol/L Ca2+; pH 7.4). Because the distribution of ions across cell membranes dramatically shifts during ischemia, the authors exposed mouse organotypic hippocampal tissue cultures to OGD in a medium, an ischemic cerebrospinal fluid, with an ion composition similar to the extracellular fluid of the brain during ischemia in vivo (70 mmol/L K+, 0.3 mmol/L Ca2+; pH 6.8). In ischemic CSF, OGD induced a selective and delayed cell death in the CA1 region, as assessed by propidium iodide uptake. Cell death was glutamate receptor dependent since blockade of the N-methyl-D-aspartate and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors mitigated cell damage. Hyperglycemia aggravates ischemic brain damage in vivo, whereas in vitro glucose in artificial CSF prevents oxygen deprivation-induced damage. The authors demonstrate that glucose in ischemic CSF significantly exacerbates cell damage after oxygen deprivation. This new model of in vitro “ischemia” can be useful in future studies of the mechanisms and treatment of ischemic cell death, including studies using genetically modified mice. |
| تدمد: | 1559-7016 0271-678X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8508c51889e16131f58023466fab3847 https://doi.org/10.1097/01.wcb.0000034361.37277.1b |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8508c51889e16131f58023466fab3847 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15597016 0271678X |
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