Modeling the effects of hyaluronic acid degradation on the regulation of human astrocyte phenotype using multicomponent interpenetrating polymer networks (mIPNs)
| العنوان: | Modeling the effects of hyaluronic acid degradation on the regulation of human astrocyte phenotype using multicomponent interpenetrating polymer networks (mIPNs) |
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| المؤلفون: | Tyler Cagle, Rachel Van Drunen, Dany J. Munoz-Pinto, Andrea C. Jimenez-Vergara |
| المصدر: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Polymers, lcsh:Medicine, Matrix (biology), Article, Extracellular matrix, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Hyaluronic acid, medicine, Humans, Hyaluronic Acid, lcsh:Science, Cells, Cultured, Inflammation, Multidisciplinary, Glial fibrillary acidic protein, biology, Chemistry, lcsh:R, Bioinspired materials, Aldehyde Dehydrogenase 1 Family, Cell biology, Extracellular Matrix, Nitric oxide synthase, 030104 developmental biology, medicine.anatomical_structure, Phenotype, Astrocytes, biology.protein, Tumor necrosis factor alpha, lcsh:Q, Astrocyte, Biomedical engineering, 030217 neurology & neurosurgery, Biomarkers |
| الوصف: | Hyaluronic acid (HA) is a highly abundant component in the extracellular matrix (ECM) and a fundamental element to the architecture and the physiology of the central nervous system (CNS). Often, HA degradation occurs when an overreactive inflammatory response, derived from tissue trauma or neurodegenerative diseases such as Alzheimer’s, causes the ECM in the CNS to be remodeled. Herein, we studied the effects of HA content as a key regulator of human astrocyte (HAf) reactivity using multicomponent interpenetrating polymer networks (mIPNs) comprised of Collagen I, HA and poly(ethylene glycol) diacrylate. The selected platform facilities the modulation of HA levels independently of matrix rigidity. Total astrocytic processes length, number of endpoints, the expression of the quiescent markers: Aldehyde Dehydrogenase 1 Family Member L1 (ALDH1L1) and Glutamate Aspartate Transporter (GLAST); the reactive markers: Glial Fibrillary Acidic Protein (GFAP) and S100 Calcium-Binding Protein β (S100β); and the inflammatory markers: Inducible Nitric Oxide Synthase (iNOS), Interleukin 1β (IL-1β) and Tumor Necrosis Factor Alpha (TNFα), were assessed. Cumulatively, our results demonstrated that the decrease in HA concentration elicited a reduction in the total length of astrocytic processes and an increase in the expression of HAf reactive and inflammatory markers. |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8534e52c92aecd1203cc96b593d252c5 https://pubmed.ncbi.nlm.nih.gov/33244148 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8534e52c92aecd1203cc96b593d252c5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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