DESIGN OF HEPATITIS E VIRUS GENOTYPE 1 RECOMBINANT CAPSID PROTEIN: CLONING, EXPRESSION, PURIFICATION, EVALUATION OF THE ANTIGENIC PROPERTIES
| العنوان: | DESIGN OF HEPATITIS E VIRUS GENOTYPE 1 RECOMBINANT CAPSID PROTEIN: CLONING, EXPRESSION, PURIFICATION, EVALUATION OF THE ANTIGENIC PROPERTIES |
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| المؤلفون: | G. I. Alatortseva, Yu. I. Ammur, K. K. Kyuregyan, V. M. Blinov, I. I. Amiantova, M. I. Mikhailov, D. S. Vorobev, O. T. Kasymov, Sidorov Av, V V Dotsenko, L. N. Luhverchik, D. A. Baiyzbekova, A. V. Milovanova, Z. Sh. Nurmatov, Zverev Vv, A. Z. Nurmatov, S. V. Zhavoronok, L. N. Nesterenko, V. Yu. Kabargina |
| المصدر: | Журнал микробиологии, эпидемиологии и иммунобиологии, Vol 0, Iss 6, Pp 72-80 (2017) |
| بيانات النشر: | Central Research Institute for Epidemiology, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, viruses, 030106 microbiology, Medicine (miscellaneous), Biology, medicine.disease_cause, Microbiology, Virus, law.invention, 03 medical and health sciences, Plasmid, hepatitis e virus, Hepatitis E virus, law, Genotype, medicine, orf2 gene, Escherichia coli, western blotting, recombinant orf2 antigen, virus diseases, General Medicine, Hepatitis E, medicine.disease, Virology, capsid protein, QR1-502, 030104 developmental biology, Capsid, Recombinant DNA, elisa, genotype 1 hev |
| الوصف: | Aim. The development of the hepatitis E virus (HEV) genotype 1 recombinant capsid protein. Materials and methods. Escherichia coli strains, plasmid vectors, serological and clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical and serological methods. Results. Using HEV genotype 1 DNA copy of subgenomic virus RNA we made E.coli strains producing recombinabt capsid protein, containing C-terminal fragment of ORF2 protein fused to E.coli beta-galactosidase. Recombinant protein ORF2 had been isolated from the inclusion bodies of the E.coli biomass and purified by size exclusion chromatography. By Western blotting it had been shown specific interaction of the recombinant polypeptide with anti-HEV IgG from pool of positive sera. Antigenic specificity of the recombinant polypeptide had been confirmed by enzyme-linked immunosorbent assay with sera of hepatitis E patients and reference groups: healthy donors, patients with hepatitis А, В, C, infectious mononucleosis and cytomegalovirus infection, HIV-infected patients. Conclusion. HEV genotype 1 ORF2 recombinant antigen had been developed, and its possible use in diagnostic tests had been experimentally shown. |
| اللغة: | Russian |
| تدمد: | 2686-7613 0372-9311 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85384315785e33a6907d753c871c54ed https://microbiol.elpub.ru/jour/article/view/233 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....85384315785e33a6907d753c871c54ed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26867613 03729311 |
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