Canalization of the urethral plate precedes fusion of the urethral folds during male penile urethral development: the double zipper hypothesis
| العنوان: | Canalization of the urethral plate precedes fusion of the urethral folds during male penile urethral development: the double zipper hypothesis |
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| المؤلفون: | Shweta Choudhry, Sisir Botta, Gerald R. Cunha, Yi Li, Joel Shen, Adriane Sinclair, Laurence S. Baskin, Mei Cao |
| المصدر: | Li, Y; Sinclair, A; Cao, M; Shen, J; Choudhry, S; Botta, S; et al.(2015). Canalization of the urethral plate precedes fusion of the urethral folds during male penile urethral development: the double zipper hypothesis.. The Journal of urology, 193(4), 1353-1359. doi: 10.1016/j.juro.2014.09.108. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/0pn5t1pt The Journal of urology, vol 193, iss 4 |
| بيانات النشر: | eScholarship, University of California, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Urologic Diseases, Male, Zipper, Urology, Organogenesis, Clinical Sciences, growth and development, Gestational Age, Optical projection tomography, Urethra, medicine, Morphogenesis, Humans, hypospadias, Process (anatomy), Urethral plate, business.industry, Anatomy, Urology & Nephrology, medicine.disease, medicine.anatomical_structure, Hypospadias, business, Groove (joinery), Penis |
| الوصف: | PurposeWe describe the "double zipper" mechanism of human male urethral formation, where the distal zipper opens the urethral groove through canalization of the urethral plate, and a second closing zipper follows behind and closes the urethral groove to form the tubular urethra.Materials and methodsAnonymous human fetal genital specimens were acquired and gender was determined by polymerase chain reaction of the Ychromosome. Specimens were processed for optical projection tomography, stained with E-cadherin, Ki67 and caspase 3, and imaged.ResultsEight developing male fetal specimens from 6.5 to 16.5 weeks of gestation were analyzed by optical projection tomography, and an additional 5specimens by serial sections. Phallus length ranged from 1.3 to 3.7 mm. The urethral plate canalized into a groove with 2 epithelial edges that subsequently fused. Ki67 staining was localized to the dorsal aspect of the urethral plate. Incontrast, caspase 3 staining was not observed. The entire process was completed during a 10-week period.ConclusionsThe human male urethra appears to form by 2 mechanisms, an initial "opening zipper" that facilitates distal canalization of the solid urethral plate to form the urethral groove, which involves a high rate of epithelial proliferation (apoptosis not observed), and a "closing zipper" facilitating fusion of the 2 epithelial surfaces of the urethral groove, and thus extending the penile urethra distally. Improved knowledge of the molecular mechanisms of these processes is critical to understanding mechanisms of abnormal urethral development, such as hypospadias. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::854aa88112444ed39aaebd0b004cf62d http://www.escholarship.org/uc/item/0pn5t1pt |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....854aa88112444ed39aaebd0b004cf62d |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |