Deconvolution of Conformational Exchange from Raman Spectra of Aqueous RNA Nucleosides
| العنوان: | Deconvolution of Conformational Exchange from Raman Spectra of Aqueous RNA Nucleosides |
|---|---|
| المؤلفون: | Paul L. A. Popelier, Jonathan P. Waltho, Andrew J. Doig, Alex L. Wilson, Carlos Outeiral, Sarah E. Dowd, Andrew Almond |
| المصدر: | Wilson, A, Outeiral Rubiera, C, Dowd, S, Doig, A, Popelier, P, Waltho, J & Almond, A 2020, ' Deconvolution of Conformational Exchange from Raman Spectra of Aqueous RNA Nucleosides ', Communications Chemistry, vol. 3, no. 56 . https://doi.org/10.1038/s42004-020-0298-x Communications Chemistry, Vol 3, Iss 1, Pp 1-9 (2020) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 010402 general chemistry, 01 natural sciences, Biochemistry, lcsh:Chemistry, chemistry.chemical_compound, symbols.namesake, Computational chemistry, 0103 physical sciences, Ribose, Materials Chemistry, Environmental Chemistry, Molecule, Conformational isomerism, Quantitative Biology::Biomolecules, Aqueous solution, 010304 chemical physics, RNA, General Chemistry, Ribonucleoside, Quantitative Biology::Genomics, 0104 chemical sciences, lcsh:QD1-999, chemistry, symbols, Deconvolution, Raman spectroscopy |
| الوصف: | Ribonucleic acids (RNAs) are key to the central dogma of molecular biology. While Raman spectroscopy holds great potential for studying RNA conformational dynamics, current computational Raman prediction and assignment methods are limited in terms of system size and inclusion of conformational exchange. Here, a framework is presented that predicts Raman spectra using mixtures of sub-spectra corresponding to major conformers calculated using classical and ab initio molecular dynamics. Experimental optimization allowed purines and pyrimidines to be characterized as predominantly syn and anti, respectively, and ribose into exchange between equivalent south and north populations. These measurements are in excellent agreement with Raman spectroscopy of ribonucleosides, and previous experimental and computational results. This framework provides a measure of ribonucleoside solution populations and conformational exchange in RNA subunits. It complements other experimental techniques and could be extended to other molecules, such as proteins and carbohydrates, enabling biological insights and providing a new analytical tool. The conformational flexibility of monoribonucleotides can make interpreting their vibrational spectra challenging. Here a combination of classical and ab initio molecular dynamics is used to calculate the contributions of different monoribonucleotide conformers to experimental Raman spectra. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85b94dcc989c1a0733b1180eea240d93 https://www.research.manchester.ac.uk/portal/en/publications/deconvolution-of-conformational-exchange-from-raman-spectra-of-aqueous-rna-nucleosides(a5757b4c-7ba0-48e6-9bf0-9f34cad56fae).html |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....85b94dcc989c1a0733b1180eea240d93 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |