A novel method for the purification of inositol phosphates from biological samples reveals that no phytate is present in human plasma or urine
| العنوان: | A novel method for the purification of inositol phosphates from biological samples reveals that no phytate is present in human plasma or urine |
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| المؤلفون: | Simon J. Bulley, Robin F. Irvine, Miranda S. C. Wilson, Adolfo Saiardi, Francesca Pisani |
| المصدر: | Open Biology Open Biology, Vol 5, Iss 3 (2015) |
| بيانات النشر: | The Royal Society, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | endocrine system, GTP', Immunology, Phosphatase, Cell, Pyrophosphate, General Biochemistry, Genetics and Molecular Biology, chemistry.chemical_compound, blood, medicine, Nucleotide, Inositol, Inositol phosphate, lcsh:QH301-705.5, chemistry.chemical_classification, IP6, biology, IP7, General Neuroscience, Research, biology.organism_classification, IP8, Dictyostelium, phytic acid, carbohydrates (lipids), medicine.anatomical_structure, chemistry, Biochemistry, lcsh:Biology (General), Research Article |
| الوصف: | Inositol phosphates are a large and diverse family of signalling molecules. While genetic studies have discovered important functions for them, the biochemistry behind these roles is often not fully characterized. A key obstacle in inositol phosphate research in mammalian cells has been the lack of straightforward techniques for their purification and analysis. Here we describe the ability of titanium dioxide (TiO2) beads to bind inositol phosphates. This discovery allowed the development of a new purification protocol that, coupled with gel analysis, permitted easy identification and quantification of InsP6(phytate), its pyrophosphate derivatives InsP7and InsP8, and the nucleotides ATP and GTP from cell or tissue extracts. Using this approach, InsP6, InsP7and InsP8were visualized inDictyosteliumextracts and a variety of mammalian cell lines and tissues, and the effects of metabolic perturbation on these were explored. TiO2bead purification also enabled us to quantify InsP6in human plasma and urine, which led to two distinct but related observations. Firstly, there is an active InsP6phosphatase in human plasma, and secondly, InsP6is undetectable in either fluid. These observations seriously question reports that InsP6is present in human biofluids and the advisability of using InsP6as a dietary supplement. |
| اللغة: | English |
| تدمد: | 2046-2441 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86058a667f1a3b6f1c8d4d2cd110f7f2 http://europepmc.org/articles/PMC4389793 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....86058a667f1a3b6f1c8d4d2cd110f7f2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20462441 |
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