Expression of Defective Hepatitis B Virus Particles Derived from Singly Spliced RNA Is Related to Liver Disease
| العنوان: | Expression of Defective Hepatitis B Virus Particles Derived from Singly Spliced RNA Is Related to Liver Disease |
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| المؤلفون: | Stanislas Pol, Jonathan Pol, Philippe Bonnard, Bertrand Nalpas, Véronique Schneider, Florianne Garreau, Patrick Soussan, Karine Lacombe, Dina Kremsdorf, Catherine Le Pendeven |
| المساهمون: | Pathogenèse des Virus de l'Hépatite B (PVHB), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5), Université Pierre et Marie Curie - Paris 6 (UPMC), Service de virologie [Hôpital Tenon], CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Département de Virologie - Department of Virology, Institut Pasteur [Paris], Service d'Immunologie [Paris], Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Département d'hépatologie [CHU Cochin], Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Cochin [AP-HP], Services des Maladies Infectieuses et Tropicales [CHU Saint-Antoine], CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service des maladies infectieuses et tropicales [CHU Tenon], Institut Cochin (UMR_S567 / UMR 8104), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale, Agence Nationale de Recherche sur le SIDA et les Hépatites Virales (grant A020042 to the study), French Research Ministry (grant to J.P.), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Pasteur [Paris] (IP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM), POL, Jonathan, Service de Maladies infectieuses et tropicales [CHU Tenon] |
| المصدر: | Journal of Infectious Diseases Journal of Infectious Diseases, Oxford University Press (OUP), 2008, 198 (2), pp.218-25. ⟨10.1086/589623⟩ Journal of Infectious Diseases, 2008, 198 (2), pp.218-25. ⟨10.1086/589623⟩ |
| بيانات النشر: | Oxford University Press (OUP), 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Liver Cirrhosis, liver diseases, viruses, dna, MESH: RNA, Viral/genetics, medicine.disease_cause, MESH: RNA Splicing, MESH: Genotype, Liver disease, 0302 clinical medicine, Orthohepadnavirus, MESH: DNA, Viral/genetics, Immunology and Allergy, rna, MESH: Hepatitis B virus/pathogenicity, MESH: Hepatitis B/genetics, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, hepatitis b virus dna, MESH: Statistics, Nonparametric, 0303 health sciences, MESH: DNA, Viral/blood, Defective Viruses, virus diseases, Viral Load, Hepatitis B, 3. Good health, Infectious Diseases, HBeAg, Hepatocellular carcinoma, Carrier State, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, RNA, Viral, 030211 gastroenterology & hepatology, MESH: Genome, Viral, MESH: Carrier State, MESH: Viral Load, Hepatitis B virus, Genotype, RNA Splicing, MESH: Inflammation/virology, MESH: Defective Viruses/pathogenicity, Genome, Viral, Biology, digestive system, Statistics, Nonparametric, Hepatitis B virus PRE beta, Virus, Necrosis, 03 medical and health sciences, MESH: Hepatitis B/virology, hepatitis b virus liver, medicine, Humans, hepatitis b virus duck, 030304 developmental biology, Inflammation, MESH: Necrosis, MESH: Humans, MESH: Liver Cirrhosis/pathology, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, medicine.disease, Virology, digestive system diseases, Hepadnaviridae, MESH: Hepatitis B virus/genetics, DNA, Viral, MESH: Defective Viruses/genetics, Immunology, MESH: Inflammation/pathology, MESH: Liver Cirrhosis/virology |
| الوصف: | Background. Defective hepatitis B virus (HBV) particles, generated from singly spliced HBV RNA, have been detected in chronic carriers of HBV. The present study was designed to quantify the expression of defective HBV (dHBV) and wild-type HBV (wtHBV) genomes in the serum of patients with HBV infection and its relation to the severity of liver disease. Methods. HBV and dHBV loads were determined by quantitative polymerase chain reaction in the serum of 89 untreatedHBV-infectedpatients(31coinfectedwithhumanimmunodeficiencyvirus[HIV]type1)withliverdisease of different stages. The ratio of dHBV DNA to total (wtHBV plus dHBV) HBV DNA (dHBV/HBV ratio) was used to express data independently of the level of viral replication. Results. DespiteaglobalcorrelationbetweendHBVandwtHBVload,thedHBV/HBVratiorangedfrom0.001% to 69%. The variation in dHBV/HBV ratio was independent of HIV coinfection, HBV genotype, and precore mutations. The mean dHBV/HBV ratio was higher in patients with severe liver necrosis and fibrosis. Conclusions. OurdataindicatethatanelevateddHBV/HBVratioisassociatedwithlivernecroinflammationand fibrosis disease, suggesting a regulation of dHBV expression according to the severity of the liver disease. The dHBV/ HBV ratio may help to better define liver disease stage during HBV infection. Persistent human hepatitis B virus (HBV) infection is a major public health problem. The major complications of chronic HBV infection are the development of liver cirrhosis and hepatocellular carcinoma (HCC) [1‐3]. Evidence indicating a direct involvement of HBV in this process is now available [3]; indeed, recent data have demonstrated that serum HBV DNA levels are strong predictorsoftheriskofHCC,independentofhepatitisB e antigen (HBeAg) expression, serum alanine amino |
| وصف الملف: | application/pdf |
| تدمد: | 1537-6613 0022-1899 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8624fb22d5e24d31185c7982d96a8ac3 https://doi.org/10.1086/589623 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8624fb22d5e24d31185c7982d96a8ac3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15376613 00221899 |
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