The mitotic kinesin-14 Ncd drives directional microtubule–microtubule sliding
| العنوان: | The mitotic kinesin-14 Ncd drives directional microtubule–microtubule sliding |
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| المؤلفون: | Cordula Reuther, Andrzej A. Kasprzak, Stefan Diez, Lukasz Hajdo, Gero Fink, Krzysztof Skowronek |
| المصدر: | Nature Cell Biology. 11:717-723 |
| بيانات النشر: | Springer Science and Business Media LLC, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Molecular Motor Proteins, Recombinant Fusion Proteins, Kinesins, Biological Transport, Spindle Apparatus, macromolecular substances, Cell Biology, Biology, Microtubule sliding, Microtubules, Spindle pole body, Spindle apparatus, Cell biology, Drosophila melanogaster, Microtubule, Animals, Drosophila Proteins, Spindle organization, Kinesin, Astral microtubules, Microtubule nucleation |
| الوصف: | During mitosis and meiosis, the bipolar spindle facilitates chromosome segregation through microtubule sliding as well as microtubule growth and shrinkage. Kinesin-14, one of the motors involved, causes spindle collapse in the absence of kinesin-5 (Refs 2, 3), participates in spindle assembly and modulates spindle length. However, the molecular mechanisms underlying these activities are not known. Here, we report that Drosophila melanogaster kinesin-14 (Ncd) alone causes sliding of anti-parallel microtubules but locks together (that is, statically crosslinks) those that are parallel. Using single molecule imaging we show that Ncd diffuses along microtubules in a tail-dependent manner and switches its orientation between sliding microtubules. Our results show that kinesin-14 causes sliding and expansion of an anti-parallel microtubule array by dynamic interactions through the motor domain on the one side and the tail domain on the other. This mechanism accounts for the roles of kinesin-14 in spindle organization. |
| تدمد: | 1476-4679 1465-7392 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::863fae422e1574d3ba616c2fa2015e6c https://doi.org/10.1038/ncb1877 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....863fae422e1574d3ba616c2fa2015e6c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14764679 14657392 |
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