Protective effect of small molecule analogues of the Acanthocheilonema viteae secreted product ES-62 on oxazolone-induced ear inflammation
| العنوان: | Protective effect of small molecule analogues of the Acanthocheilonema viteae secreted product ES-62 on oxazolone-induced ear inflammation |
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| المؤلفون: | Miguel A. Pineda, Lamyaa Al-Riyami, Margaret M. Harnett, Colin J. Suckling, William Harnett, David T. Rodgers, Justyna Rzepecka |
| المصدر: | Experimental Parasitology |
| بيانات النشر: | Elsevier BV, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Immunology, Inflammation, Pharmacology, Real-Time Polymerase Chain Reaction, RS, Immunomodulation, Oxazolone, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Adjuvants, Immunologic, Full Length Article, otorhinolaryngologic diseases, medicine, Acanthocheilonema, Animals, Allergic contact dermatitis, 030304 developmental biology, Mice, Inbred BALB C, 0303 health sciences, Acanthocheilonema viteae, biology, Phosphorylcholine, Skin inflammation, Helminth Proteins, General Medicine, Otitis Externa, biology.organism_classification, Mast cell, medicine.disease, 3. Good health, Cellular infiltration, Disease Models, Animal, ES-62, Infectious Diseases, medicine.anatomical_structure, chemistry, 030220 oncology & carcinogenesis, QR180, Dermatitis, Allergic Contact, Parasitology, medicine.symptom, Parasitic worm |
| الوصف: | Highlights • Small molecule analogues (SMAs) of the immunomodulator, ES-62, have been produced. • Two SMAs protect against oxazolone-induced skin inflammation in mouse ears. • Protection is associated with reduced cellular infiltration and collagen deposition. • Protection is associated with decreased IFNγ mRNA in the ears. Graphical Abstract ES-62 is the major secreted protein of the rodent filarial nematode Acanthocheilonema viteae. The molecule contains covalently attached phosphorylcholine (PC) residues, which confer anti-inflammatory properties on ES-62, underpinning the idea that drugs based on this active moiety may have therapeutic potential in human diseases associated with aberrant inflammation. Here we demonstrate that two synthetic small molecule analogues (SMAs) of ES-62 termed SMA 11a and SMA 12b are protective in the oxazolone-induced acute allergic contact dermatitis mouse model of skin inflammation, as measured by a significant reduction in ear inflammation following their administration before oxazolone sensitisation and before oxazolone challenge. Furthermore, it was found that when tested, 12b was effective at reducing ear swelling even when first administered before challenge. Histological analysis of the ears showed elevated cellular infiltration and collagen deposition in oxazolone-treated mice both of which were reduced by treatment with the two SMAs. Likewise, the oxazolone-induced increase in IFNγ mRNA in the ears was reduced but no effect on other cytokines investigated was observed. Finally, no influence on the mast cell populations in the ear was observed. |
| وصف الملف: | application/pdf |
| تدمد: | 0014-4894 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8646953c8af51b709bdf5223bcda2984 https://doi.org/10.1016/j.exppara.2015.03.025 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8646953c8af51b709bdf5223bcda2984 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00144894 |
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