Cardiac Myocyte Diversity and a Fibroblast Network in the Junctional Region of the Zebrafish Heart Revealed by Transmission and Serial Block-Face Scanning Electron Microscopy
| العنوان: | Cardiac Myocyte Diversity and a Fibroblast Network in the Junctional Region of the Zebrafish Heart Revealed by Transmission and Serial Block-Face Scanning Electron Microscopy |
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| المؤلفون: | Norman Hu, Pascal J. Lafontant, Evelyn Brown, Paul Landry, Alan R. Burns, Ali Reza Behzad |
| المصدر: | PLoS ONE PLoS ONE, Vol 8, Iss 8, p e72388 (2013) |
| بيانات النشر: | Public Library of Science, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Serial block-face scanning electron microscopy, Anatomy and Physiology, lcsh:Medicine, Cell junction, Cardiovascular System, 0302 clinical medicine, Desmosome, Molecular Cell Biology, Morphogenesis, Myocyte, Myocytes, Cardiac, Comparative Anatomy, lcsh:Science, Zebrafish, Connective Tissue Cells, 0303 health sciences, education.field_of_study, Multidisciplinary, biology, Heart development, Chemistry, Cardiac myocyte, Anatomy, Animal Models, Cell biology, Extracellular Matrix, medicine.anatomical_structure, Connective Tissue, Heart Development, Collagen, Cellular Types, Research Article, Population, 03 medical and health sciences, Model Organisms, Microscopy, Electron, Transmission, medicine, Regeneration, Animals, education, Biology, 030304 developmental biology, Myocytes, Myocardium, lcsh:R, Endothelial Cells, Fibroblasts, biology.organism_classification, Extracellular Matrix Composition, Cardiovascular Anatomy, Microscopy, Electron, Scanning, lcsh:Q, Organism Development, Zoology, 030217 neurology & neurosurgery, Developmental Biology |
| الوصف: | The zebrafish has emerged as an important model of heart development and regeneration. While the structural characteristics of the developing and adult zebrafish ventricle have been previously studied, little attention has been paid to the nature of the interface between the compact and spongy myocardium. Here we describe how these two distinct layers are structurally and functionally integrated. We demonstrate by transmission electron microscopy that this interface is complex and composed primarily of a junctional region occupied by collagen, as well as a population of fibroblasts that form a highly complex network. We also describe a continuum of uniquely flattened transitional cardiac myocytes that form a circumferential plate upon which the radially-oriented luminal trabeculae are anchored. In addition, we have uncovered within the transitional ring a subpopulation of markedly electron dense cardiac myocytes. At discrete intervals the transitional cardiac myocytes form contact bridges across the junctional space that are stabilized through localized desmosomes and fascia adherentes junctions with adjacent compact cardiac myocytes. Finally using serial block-face scanning electron microscopy, segmentation and volume reconstruction, we confirm the three-dimensional nature of the junctional region as well as the presence of the sheet-like fibroblast network. These ultrastructural studies demonstrate the previously unrecognized complexity with which the compact and spongy layers are structurally integrated, and provide a new basis for understanding development and regeneration in the zebrafish heart. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8686440cec711501f9fe42e402044842 http://europepmc.org/articles/PMC3751930 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8686440cec711501f9fe42e402044842 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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