Mechanism and Kinetics of Lipid Bilayer Formation in Solid-State Nanopores
| العنوان: | Mechanism and Kinetics of Lipid Bilayer Formation in Solid-State Nanopores |
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| المؤلفون: | Zhen Zhang, Daniel Aili, Johanna Utterström, Robert Selegård, Chenyu Wen, Shi-Li Zhang, Shuangshuang Zeng, Shiyu Li |
| المصدر: | Langmuir. 36:1446-1453 |
| بيانات النشر: | American Chemical Society (ACS), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Analyte, Materials science, Kinetics, Solid-state, food and beverages, 02 engineering and technology, Surfaces and Interfaces, engineering.material, 010402 general chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 01 natural sciences, 0104 chemical sciences, Nanopore, Chemical engineering, Coating, Electrochemistry, engineering, Molecule, General Materials Science, 0210 nano-technology, Lipid bilayer, Spectroscopy, Mechanism (sociology) |
| الوصف: | Solid-state nanopores provide a highly versatile platform for rapid electrical detection and analysis of single molecules. Lipid bilayer coating of the nanopores can reduce nonspecific analyte adsorption to the nanopore sidewalls and increase the sensing selectivity by providing possibilities for tethering specific ligands in a cell-membrane mimicking environment. However, the mechanism and kinetics of lipid bilayer formation from vesicles remain unclear in the presence of nanopores. In this work, we used a silicon-based, truncated pyramidal nanopore array as the support for lipid bilayer formation. Lipid bilayer formation in the nanopores was monitored in real time by the change in ionic current through the nanopores. Statistical analysis revealed that a lipid bilayer is formed from the instantaneous rupture of individual vesicle upon adsorption in the nanopores, differing from the generally agreed mechanism that lipid bilayer forms at a high vesicle surface coverage on a planar support. The dependence of the lipid bilayer formation process on the applied bias, vesicle size, and concentration was systematically studied. In addition, the nonfouling properties of the lipid bilayer coated nanopores were demonstrated during long single-stranded DNA translocation through the nanopore array. The findings indicate that the lipid bilayer formation process can be modulated by introducing nanocavities intentionally on the planar surface to create active sites or changing the vesicle size and concentration. |
| تدمد: | 1520-5827 0743-7463 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::869126e2a5632d868b2d806f0a84e4f5 https://doi.org/10.1021/acs.langmuir.9b03637 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....869126e2a5632d868b2d806f0a84e4f5 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15205827 07437463 |
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