Divergence of three BRX homoeologs in Brassica rapa and its effect on leaf morphology
| العنوان: | Divergence of three BRX homoeologs in Brassica rapa and its effect on leaf morphology |
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| المؤلفون: | Jianli Liang, Runmao Lin, Feng Cheng, Xu Cai, Jian Wu, Xiaowu Wang, Yuanyuan Zhang, Haixu Chen |
| المصدر: | Horticulture Research |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Tiller (botany), Plant Science, Horticulture, Biology, 01 natural sciences, Biochemistry, Article, Petiole (botany), 03 medical and health sciences, Arabidopsis, Brassica rapa, Genetics, Gene family, Leafy, Leaf development, fungi, food and beverages, biology.organism_classification, Phenotype, Complementation, Polyploidy in plants, 030104 developmental biology, 010606 plant biology & botany, Biotechnology |
| الوصف: | The leafy head characteristic is a special phenotype of Chinese cabbage resulting from artificial selection during domestication and breeding. BREVIS RADIX (BRX) has been suggested to control root elongation, shoot growth, and tiller angle in Arabidopsis and rice. In Brassica rapa, three BrBRX homoeologs have been identified, but only BrBRX.1 and BrBRX.2 were found to be under selection in leaf-heading accessions, indicating their functional diversification in leafy head formation. Here, we show that these three BrBRX genes belong to a plant-specific BRX gene family but that they have significantly diverged from other BRX-like members on the basis of different phylogenetic classifications, motif compositions and expression patterns. Moreover, although the expression of these three BrBRX genes differed, compared with BrBRX.3, BrBRX.1, and BrBRX.2 displayed similar expression patterns. Arabidopsis mutant complementation studies showed that only BrBRX.1 could rescue the brx root phenotype, whereas BrBRX.2 and BrBRX.3 could not. However, overexpression of each of the three BrBRX genes in Arabidopsis resulted in similar pleiotropic leaf phenotypes, including epinastic leaf morphology, with an increase in leaf number and leaf petiole length and a reduction in leaf angle. These leaf traits are associated with leafy head formation. Further testing of a SNP (T/C) in BrBRX.2 confirmed that this allele in the heading accessions was strongly associated with the leaf-heading trait of B. rapa. Our results revealed that all three BrBRX genes may be involved in the leaf-heading trait, but they may have functionally diverged on the basis of their differential expression. |
| تدمد: | 2052-7276 2662-6810 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86ccf117ce3c586756a9c8f823555224 https://doi.org/10.1038/s41438-021-00504-3 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....86ccf117ce3c586756a9c8f823555224 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20527276 26626810 |
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