Quantitative assessment of gene promoter methylation in non-small cell lung cancer using methylation-sensitive high-resolution melting
| العنوان: | Quantitative assessment of gene promoter methylation in non-small cell lung cancer using methylation-sensitive high-resolution melting |
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| المؤلفون: | Qiqing Zhang, Honglian Zhang, Hongju Mao, Shaohua Lu, Yanan Bai, Zhenhua Wu, Zule Cheng, Zhao Jianlong, Fangming Liu, Lin Zhou |
| المصدر: | Oncology Letters |
| بيانات النشر: | D.A. Spandidos, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Methyltransferase, high-resolution melting, diagnosis, Bisulfite sequencing, Promoter, Methylation, Articles, Biology, High Resolution Melt, 03 medical and health sciences, lung cancer, 030104 developmental biology, 0302 clinical medicine, Oncology, 030220 oncology & carcinogenesis, CpG, DNA methylation, microRNA, Cancer research, Epigenetics, methylation |
| الوصف: | DNA methylation is closely associated with aberrant epigenetic changes. Previous studies have identified various genes associated with non-small cell lung cancer (NSCLC), but the precise combination responsible for its etiology is still debated. The aim of the present study was to select a new set of NSCLC-related genes using methylation-sensitive high-resolution melting. The promoter methylation status of six selected genes, consisting of protocadherin γ subfamily B, 6 (PCDHGB6), homeobox A9 (HOXA9), O6-methylguanine-DNA methyltransferase (MGMT), microRNA (miR)-126, suppressor of cytokine signaling 3 (SOCS3) and Ras association domain family member 5, also termed NORE1A, was evaluated in 54 NSCLC patients. From these samples, genome-wide DNA was extracted and bisulfite conversion was performed along with fluorogenic quantitative polymerase chain reaction to detect methylation values of the six selected promoters. The present results revealed frequent methylation on PCDHGB6, HOXA9 and miR-126, which contrasted with infrequent methylation on MGMT. The results indicated no methylation on either SOCS3 or NORE1A. The sensitivity and specificity of the methylation assessment were 85.2 and 81.5%, respectively, and the analysis results were validated by pyrosequencing. Furthermore, minute comparison of the association between DNA methylation and clinical features was performed. Overall, these results may provide potential information for the development of better clinical diagnostics and more targeted and effective therapies for NSCLC. |
| اللغة: | English |
| تدمد: | 1792-1082 1792-1074 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::870cbdde023ac145d4c452e7b98c267a http://europepmc.org/articles/PMC5920472 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....870cbdde023ac145d4c452e7b98c267a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921082 17921074 |
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