Elevated μ-opioid receptor expression in the nucleus of the solitary tract accompanies attenuated withdrawal signs after chronic low dose naltrexone in opiate-dependent rats
العنوان: | Elevated μ-opioid receptor expression in the nucleus of the solitary tract accompanies attenuated withdrawal signs after chronic low dose naltrexone in opiate-dependent rats |
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المؤلفون: | Y. Qian, V.C. Oropeza, Paolo Mannelli, E.J. Van Bockstaele, C. Rudoy |
المصدر: | Journal of Neuroscience Research. 83:508-514 |
بيانات النشر: | Wiley, 2006. |
سنة النشر: | 2006 |
مصطلحات موضوعية: | Male, Narcotics, medicine.medical_specialty, Time Factors, medicine.drug_class, Narcotic Antagonists, Blotting, Western, Receptors, Opioid, mu, Gene Expression, Striatum, Pharmacology, CREB, Naltrexone, Rats, Sprague-Dawley, Cellular and Molecular Neuroscience, Prosencephalon, Opioid receptor, Internal medicine, Solitary Nucleus, medicine, Animals, Drug Interactions, Behavior, Animal, Dose-Response Relationship, Drug, Morphine, biology, Chemistry, Drug Administration Routes, Solitary tract, CREB-Binding Protein, Cyclic AMP-Dependent Protein Kinases, Rats, Substance Withdrawal Syndrome, Intracellular signal transduction, Ventral tegmental area, Disease Models, Animal, Endocrinology, medicine.anatomical_structure, biology.protein, Low-dose naltrexone, medicine.drug |
الوصف: | We previously described a decrease in withdrawal behaviors in opiate-dependent rats that were chronically treated with very low doses of naltrexone in their drinking water. Attenuated expression of withdrawal behaviors correlated with decreased c-Fos expression and intracellular signal transduction elements [protein kinase A regulatory subunit II (PKA) and phosphorylated cAMP response element binding protein (pCREB)] in brainstem noradrenergic nuclei. In this study, to determine whether similar cellular changes occurred in forebrain nuclei associated with drug reward, expressions of PKA and pCREB were analyzed in the ventral tegmental area, frontal cortex, striatum, and amygdala of opiate-treated rats that received low doses of naltrexone in their drinking water. No significant difference in PKA or pCREB was detected in these regions following drug treatment. To examine further the cellular mechanisms in noradrenergic nuclei that could underlie attenuated withdrawal behaviors following low dose naltrexone administration, the nucleus of the solitary tract (NTS) and locus coeruleus (LC) were examined for opioid receptor (OR) protein expression. Results showed a significant increase in μOR expression in the NTS of morphine-dependent rats that received low doses of naltrexone in their drinking water, and increases in μOR expression were also found to be dose dependent. Protein expression of μOR in the LC and δOR in either brain region remained unchanged. In conclusion, our previously reported decreases in c-Fos and PKA expression in the NTS following pretreatment with low doses of naltrexone may be partially explained by a greater inhibition of NTS neurons resulting from increased μOR expression in this region. © 2005 Wiley-Liss, Inc. |
تدمد: | 1097-4547 0360-4012 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::870d540a12808459f3b91ff589c78e37 https://doi.org/10.1002/jnr.20738 |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi.dedup.....870d540a12808459f3b91ff589c78e37 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 10974547 03604012 |
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