A novel in vitro model for the assessment of postnatal myonuclear accretion
| العنوان: | A novel in vitro model for the assessment of postnatal myonuclear accretion |
|---|---|
| المؤلفون: | Ellis Gielen, Anita Kneppers, Luc J. C. van Loon, Chiel C. de Theije, Ramon C. J. Langen, Mark Corten, Annemie M. W. J. Schols, Lex B. Verdijk |
| المساهمون: | Pulmonologie, RS: NUTRIM - R3 - Respiratory & Age-related Health, Promovendi NTM, Humane Biologie, Ondersteunend personeel NTM |
| المصدر: | Skeletal Muscle, Vol 8, Iss 1, Pp 1-13 (2018) Skeletal Muscle Skeletal Muscle, 8:4. BioMed Central Ltd |
| بيانات النشر: | BioMed Central, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, postnatal myogenesis, MUSCLE STEM-CELLS, lcsh:Diseases of the musculoskeletal system, MYOTUBE HYPERTROPHY, Cellular differentiation, Muscle Fibers, Skeletal, Interleukin-4/pharmacology, Cell, Muscle Proteins, Myostatin, Muscle Development, Mice, Myoblast fusion, FIBER HYPERTROPHY, Models, muscle repair, Myocyte, Orthopedics and Sports Medicine, Insulin-Like Growth Factor I, Muscle Development/drug effects, Cells, Cultured, Muscle Proteins/metabolism, satellite cells, muscle regeneration, Interleukin-13, Cultured, biology, Myogenesis, myoblasts, Muscle Fibers, Skeletal/cytology, REGENERATIVE MYOGENESIS, HUMAN SKELETAL-MUSCLE, TNF-ALPHA, Cell biology, medicine.anatomical_structure, MYOBLAST FUSION, Skeletal/cytology, DIGITORUM LONGUS MUSCLE, Insulin-Like Growth Factor I/pharmacology, Muscle, C2C12, Signal Transduction, Interleukin-13/pharmacology, Regeneration/drug effects, Cells, Models, Biological, Muscle Fibers, 03 medical and health sciences, Skeletal/physiology, medicine, Regeneration, Humans, Animals, skeletal muscle, Muscle, Skeletal, Molecular Biology, cell fusion, Signal Transduction/physiology, Methodology, Membrane Proteins, Skeletal muscle, Cell Biology, Biological, muscle maintenance, Coculture Techniques, Muscle, Skeletal/physiology, cell differentiation, 030104 developmental biology, myotubes, Cell Differentiation/drug effects, biology.protein, Membrane Proteins/metabolism, Interleukin-4, lcsh:RC925-935, GAMMA-IRRADIATION, Atrophy, Myoblasts/cytology |
| الوصف: | Background Due to the post-mitotic nature of myonuclei, postnatal myogenesis is essential for skeletal muscle growth, repair, and regeneration. This process is facilitated by satellite cells through proliferation, differentiation, and subsequent fusion with a pre-existing muscle fiber (i.e., myonuclear accretion). Current knowledge of myogenesis is primarily based on the in vitro formation of syncytia from myoblasts, which represents aspects of developmental myogenesis, but may incompletely portray postnatal myogenesis. Therefore, we aimed to develop an in vitro model that better reflects postnatal myogenesis, to study the cell intrinsic and extrinsic processes and signaling involved in the regulation of postnatal myogenesis. Methods Proliferating C2C12 myoblasts were trypsinized and co-cultured for 3 days with 5 days differentiated C2C12 myotubes. Postnatal myonuclear accretion was visually assessed by live cell time-lapse imaging and cell tracing by cell labeling with Vybrant® DiD and DiO. Furthermore, a Cre/LoxP-based cell system was developed to semi-quantitatively assess in vitro postnatal myonuclear accretion by the conditional expression of luciferase upon myoblast–myotube fusion. Luciferase activity was assessed luminometrically and corrected for total protein content. Results Live cell time-lapse imaging, staining-based cell tracing, and recombination-dependent luciferase activity, showed the occurrence of postnatal myonuclear accretion in vitro. Treatment of co-cultures with the myogenic factor IGF-I (p |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2044-5040 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87156d3fc968b0798d2685a4c5e60989 https://acuresearchbank.acu.edu.au/item/8845w/a-novel-in-vitro-model-for-the-assessment-of-postnatal-myonuclear-accretion |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....87156d3fc968b0798d2685a4c5e60989 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20445040 |
|---|