Different mechanisms of Campath-1H-mediated depletion for CD4+and CD8+T cells in peripheral blood
| العنوان: | Different mechanisms of Campath-1H-mediated depletion for CD4+and CD8+T cells in peripheral blood |
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| المؤلفون: | Alan Chant, Hayden Lowenstein, Abrar Khan, Akeesha Shah |
| المصدر: | Transplant International. 19:927-936 |
| بيانات النشر: | Frontiers Media SA, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, Antibodies, Neoplasm, T-Lymphocytes, Ficoll, Cell Separation, CD8-Positive T-Lymphocytes, Antibodies, Monoclonal, Humanized, Ammonium Chloride, Blood cell, Antigen, Antigens, CD, Antigens, Neoplasm, medicine, Humans, Cytotoxic T cell, Lymphocytes, Alemtuzumab, Glycoproteins, Whole blood, Transplantation, Cell Death, Dose-Response Relationship, Drug, biology, business.industry, Antibodies, Monoclonal, T lymphocyte, Flow Cytometry, medicine.anatomical_structure, CD52 Antigen, Immunology, Leukocytes, Mononuclear, biology.protein, Antibody, business, Immunosuppressive Agents, CD8 |
| الوصف: | It is assumed that complement and noncomplement-mediated mechanisms are similarly responsible for Campath-1H-mediated killing of all T-cell subtypes in vivo. However, the differing surface expression of CD52 on T-cell subtypes suggests that may not be the case. The purpose of this study is to determine the extent and mechanism of Campath-1H-mediated elimination of different T-cell subtypes in peripheral blood. Whole blood or lymphocytes isolated from peripheral blood of healthy volunteers by Ficoll density centrifugation were incubated with Campath-1H, with or without complement and/or serum, and the resultant T-cell elimination mechanisms studied. For CD4(+) T lymphocytes, 60% and 40% cell death and for CD8(+) T lymphocytes 23% and 77% cell death, in peripheral blood, was mediated by complement and noncomplement mediated mechanisms, respectively. CD4(+) T cells demonstrated approximately twice the amount of surface CD52 compared with CD8(+) T cells, consistent with primarily complement-mediated killing for CD4(+) T cells. Thus, peripheral blood supports differential and partial elimination of T-cell subtypes, suggesting that the complete T-cell elimination seen in transplant recipients is most likely due to contribution from other lymphoid organs. |
| تدمد: | 1432-2277 0934-0874 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::871df3ab15c67f5e1d6d76a321e2ab53 https://doi.org/10.1111/j.1432-2277.2006.00382.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....871df3ab15c67f5e1d6d76a321e2ab53 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14322277 09340874 |
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