Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene
العنوان: | Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene |
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المؤلفون: | Marcelo Elhers Loureiro, Ana Paula Gallina, Giovani Greigh de Brito, Eunize Maciel Zambolim, Valdir Diola, Eveline Teixeira Caixeta, Laércio Zambolim |
المساهمون: | GIOVANI GREIGH DE BRITO, CNPA, EVELINE TEIXEIRA CAIXETA, SAPC, ANA PAULA GALLINA, Universidade Federal de Viçosa, EUNIZE MACIEL ZAMBOLIM, Universidade Federal de Viçosa, LAÉRCIO ZAMBOLIM, Universidade Federal de Viçosa, VALDIR DIOLA, Universidade Federal de Viçosa, MARCELO ELHERS LOUREIRO, Universidade Federal de Viçosa. |
المصدر: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
بيانات النشر: | Springer Science and Business Media LLC, 2010. |
سنة النشر: | 2010 |
مصطلحات موضوعية: | Hemileia vastatrix, Genetics, biology, Bulked segregant analysis, Resistance gene, Plant Science, Coffee leaf rust, Horticulture, Marker-assisted selection, Plant disease resistance, biology.organism_classification, Gene mapping, Genetic marker, Genotype, AFLP marker, Amplified fragment length polymorphism, Agronomy and Crop Science |
الوصف: | The most important disease of Coffea arabica is coffee leaf rust caused by the fungus Hemileia vastatrix. The purpose of this study was to characterize the inheritance of coffee resistance gene(s) to race II of this pathogen and to identify and map molecular markers linked to this trait. Different populations were used: F2 (160 plants), BCr (20), and BCs (135), derived from a cross between the resistant genotype Hı´brido de Timor UFV 427-15 and the susceptible cultivar Catuaı´ Amarelo UFV 2143-236 (IAC 30). The segregation analysis showed that the resistance of Hı´brido de Timor to race II of the H. vastatrix is conferred by a single dominant gene. The amplification of 176 AFLP (Amplified fragment length polymorphism) primer combinations using bulked segregant analysis (BSA) allowed the identification of three molecular markers linked to the resistance gene. Genetic mapping of these three markers in the F2 population indicated that they are distributed on both sides, flanking the resistance gene. The markers E.CTC/M.TTT405 and E.CGT/M.TGT 300 were found linked to the resistance gene at 8.69 cM (LOD 18.91) and 25.10 cM (LOD 5.37), respectively, while E.CCT/M.TTC230 was localized on the other side of the gene, at 20.50 cM (LOD 6.15). These markers are the first rust resistance markers identified in Hı´brido de Timor and can be useful for marker assisted selection in coffee breeding programs. |
وصف الملف: | application/pdf |
تدمد: | 1573-5060 0014-2336 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87a2e37e7847d963887d8f34c07f49b7 https://doi.org/10.1007/s10681-010-0119-x |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....87a2e37e7847d963887d8f34c07f49b7 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 15735060 00142336 |
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