Expression and structural features of endoglin (CD105), a transforming growth factor beta 1 and beta 3 binding protein, in human melanoma
| العنوان: | Expression and structural features of endoglin (CD105), a transforming growth factor beta 1 and beta 3 binding protein, in human melanoma |
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| المؤلفون: | P. G. Natali, Maria Rita Nicotra, Maresa Altomonte, Michele Maio, I. Cattarossi, Francesca Colizzi, Ester Fonsatti, L. I. Brasoveanu, R. Montagner, A. Cattelan |
| المصدر: | British Journal of Cancer Scopus-Elsevier |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Cancer Research, medicine.medical_specialty, Skin Neoplasms, medicine.medical_treatment, Disuccinimidyl suberate, Vascular Cell Adhesion Molecule-1, Receptors, Cell Surface, Biology, Skin Diseases, Iodine Radioisotopes, chemistry.chemical_compound, Mice, Antigen, Antigens, CD, Transforming Growth Factor beta, Internal medicine, medicine, Tumor Cells, Cultured, Animals, Humans, Fluorescent Antibody Technique, Indirect, Melanoma, Nevus, Mice, Inbred BALB C, Binding protein, Endoglin, Transforming growth factor beta, medicine.disease, Molecular biology, Immunohistochemistry, Neoplasm Proteins, Endocrinology, Cytokine, Oncology, chemistry, biology.protein, Electrophoresis, Polyacrylamide Gel, Cell Division, Transforming growth factor, Research Article |
| الوصف: | Human endoglin (CD105) is a member of the transforming growth factor beta (TGF-beta) receptor family that binds TGF-beta1 and -beta3, but not TGF-beta2, on human endothelial cells. Immunohistochemical analyses demonstrated that CD105 is expressed on normal and neoplastic cells of the melanocytic lineage. The anti-CD105 MAb, MAEND3, stained 50, 25 and 34% of intradermal naevi, primary and metastatic melanomas investigated, respectively, and nine out of 12 melanoma cell lines. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that CD105 expressed by melanoma cells consists of a homodimeric protein with an apparent molecular weight of 180 and 95 kDa under non-reducing and reducing conditions. Cross-linking of 125I-labelled TGF-beta1 to melanoma cells, Mel 97, by disuccinimidyl suberate (DSS) demonstrated that CD105 expressed on pigmented cells binds TGF-beta1; the pattern of binding of TGF-beta1 to melanoma cells was found to be similar to that of human umbilical vein endothelial cells. The addition of exogenous, bioactive TGF-beta1 significantly (P |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87da13ab805bf7383b9635dadda83483 http://hdl.handle.net/11365/1081376 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....87da13ab805bf7383b9635dadda83483 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |