SARS-CoV-2 integral membrane proteins shape the serological responses of patients with COVID-19
| العنوان: | SARS-CoV-2 integral membrane proteins shape the serological responses of patients with COVID-19 |
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| المؤلفون: | Vincent Thibault, Leif A. Eriksson, Gwénaële Jégou, Tony Avril, Eric Chevet, Florence Godey, Matthieu Le Gallo, Christopher Heslan, Sophie Martin |
| المصدر: | iScience iScience, Vol 24, Iss 10, Pp 103185-(2021) |
| بيانات النشر: | The Author(s)., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0303 health sciences, Multidisciplinary, Coronavirus disease 2019 (COVID-19), Membrane insertion, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Science, viruses, Immunology, Biology, biochemical phenomena, metabolism, and nutrition, Virology, Transmembrane protein, Article, 3. Good health, Serology, 03 medical and health sciences, 0302 clinical medicine, Immune system, In patient, Integral membrane protein, 030217 neurology & neurosurgery, 030304 developmental biology |
| الوصف: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has elicited a unique mobilization of the scientific community to develop efficient tools to understand and combat the infection. Like other coronavirae, SARS-CoV-2 hijacks host cell secretory machinery to produce viral proteins that compose the nascent virions; including spike (S), envelope (E), and membrane (M) proteins, the most exposed transmembrane proteins to the host immune system. As antibody response is part of the anti-viral immune arsenal, we investigate the immunogenic potential of S, E, and M using a human cell-based system to mimic membrane insertion and N-glycosylation. Both S and M elicit specific Ig production in patients with SARS-CoV-2. Patients with moderate and severe diseases exhibit elevated Ig responses. Finally, reduced Ig binding was observed with spike G614 compared to D614 variant. Altogether, our assay points toward an unexpected immune response against M and represents a powerful tool to test humoral responses against actively evolving SARS-CoV-2 variants and vaccine effectiveness. Graphical abstract Immunology; Virology |
| اللغة: | English |
| تدمد: | 2589-0042 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87db4f1821565c082cec060b1054d50e http://europepmc.org/articles/PMC8479324 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....87db4f1821565c082cec060b1054d50e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 25890042 |
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