Investigation of the Subcellular Localization-Dependent Anti- or Pro-Tumor Functions of Maspin in Human Lung Adenocarcinoma Cell Line
| العنوان: | Investigation of the Subcellular Localization-Dependent Anti- or Pro-Tumor Functions of Maspin in Human Lung Adenocarcinoma Cell Line |
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| المؤلفون: | Takahiro, Matsushige, Tomohiko, Sakabe, Yoshihisa, Umekita |
| المصدر: | Yonago Acta Med Yonago Acta Medica. 2022, 65(1), 44-52. doi:10.33160/yam.2022.02.006 |
| بيانات النشر: | Tottori University Medical Press, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Original Article, General Medicine, maspin, lung adenocarcinoma |
| الوصف: | BACKGROUND: Mammary serine protease inhibitor (maspin) is well known as a tumor suppressor gene in several types of cancers and its nuclear localization is essential for its tumor-suppressive function. We previously reported that the cytoplasmic-only localization of maspin is significantly correlated with unfavorable prognosis in patients with lung adenocarcinoma (LUAD). To clarify whether maspin in LUAD acts as a tumor promoter or suppressor, we examined the subcellular localization-dependent biological functions of maspin in human LUAD cell lines. METHODS: The expression levels and subcellular localization of maspin were investigated by performing immunoblotting and immunofluorescence in human LUAD cell lines (PC-9, A549, NCI-H23, RERF-LC-KJ) and human bronchial epithelial cell line (BEAS-2B). We then established stable cell lines overexpressing maspin (A549-maspin and RERF-LC-KJ-maspin) and investigated their subcellular localization. Cell invasion assays of these cell lines were performed to examine their invasiveness. Moreover, the mRNA expression levels between epithelial cell markers (E-cadherin) and mesenchymal cell markers (N-cadherin and vimentin) were compared. RESULTS: The expression of maspin in PC-9 cells was comparable to that in BEAS-2B cells, whereas its expression in A549, NCI-H23, and RERF-LC-KJ cells was decreased. The cell invasion capability of A549-maspin cells showing pancellular expression was significantly decreased compared with that of A549-control cells. By contrast, the cell invasion capability of RERF-LC-KJ-maspin cells showing cytoplasmic-only expression was significantly increased compared with that of RERF-LC-KJ-control cells. The mRNA expression levels of N-cadherin, but not E-cadherin and vimentin, in A549-maspin cells was significantly downregulated compared with that in A549-control cells. No significant differences in these markers were observed between RERF-LC-KJ-maspin and RERF-LC-KJ-control cells. CONCLUSION: The invasive capability of LUAD cells is regulated by the intracellular localization of maspin. Clarification of the molecular mechanism underlying the subcellular localization-dependent function of maspin will promote a deeper understanding of LUAD development and progression. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0513-5710 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87e167c4e8631fbec339c225a5422064 http://repository.lib.tottori-u.ac.jp/13696 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....87e167c4e8631fbec339c225a5422064 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 05135710 |
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