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Protection against Streptococcus pneumoniae serotype 1 acute infection shows a signature of Th17- and IFN-γ-mediated immunity

التفاصيل البيبلوغرافية
العنوان: Protection against Streptococcus pneumoniae serotype 1 acute infection shows a signature of Th17- and IFN-γ-mediated immunity
المؤلفون: Juan Martín Marqués, Jean-Claude Sirard, Teresa Camou, François-Xavier Pellay, Analía Rial, Hélène Léger, Arndt Benecke, Laurye Van Maele, José A. Chabalgoity, Natalia Muñoz
المساهمون: Laboratory for Vaccine Research (LVR), Instituto de Higiene, Facultad de Medicina, Universidad de la Republica Oriental, Institut des Hautes Études Scientifiques (IHES), IHES, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Departamento de Laboratorios del Ministerio de Salud Pública, Ministerio de Salud Publica, ECOS-Sud (U08S02), Institut des Hautes Etudes Scientifiques (IHES), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Sirard, Jean-Claude
المصدر: Immunobiology
Immunobiology, 2012, 217 (4), pp.420-9. ⟨10.1016/j.imbio.2011.10.012⟩
Immunobiology, Elsevier, 2012, 217 (4), pp.420-9. ⟨10.1016/j.imbio.2011.10.012⟩
بيانات النشر: HAL CCSD, 2012.
سنة النشر: 2012
مصطلحات موضوعية: Neutrophils, medicine.medical_treatment, MESH: Th17 Cells, MESH: Interleukin-17, MESH: Neutrophils, medicine.disease_cause, Mice, 0302 clinical medicine, MESH: Antibodies, Bacterial, MESH: Up-Regulation, Immunology and Allergy, MESH: Animals, MESH: Pneumococcal Infections, MESH: Polysaccharides, Bacterial, Lung, 0303 health sciences, Interleukin-17, Polysaccharides, Bacterial, Hematology, Antibodies, Bacterial, Up-Regulation, 3. Good health, Streptococcus pneumoniae, Cytokine, Pneumococcal pneumonia, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, Antibody, MESH: Streptococcus pneumoniae, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH: Interferon-gamma, Immunology, Biology, Pneumococcal Infections, Microbiology, Interferon-gamma, 03 medical and health sciences, Downregulation and upregulation, Immunity, MESH: Mice, Inbred C57BL, medicine, Animals, Humans, MESH: Lung, MESH: Mice, MESH: Humans, 030306 microbiology, Lethal dose, Microarray Analysis, medicine.disease, Mice, Inbred C57BL, Disease Models, Animal, Pneumonia, MESH: Microarray Analysis, MESH: Cytoprotection, Cytoprotection, biology.protein, Th17 Cells, MESH: Disease Models, Animal, MESH: Female, 030215 immunology
الوصف: International audience; Acute pneumonia caused by Streptococcus pneumoniae is a major cause of child mortality. Antibodies are considered the main effectors of protection in this clinical presentation of pneumococcal invasive disease. To get new insights into the mechanisms involved in the protective immunity, we established a murine experimental model of protection against acute pneumococcal pneumonia and then evaluated the transcriptional, humoral and cellular responses in protected and non-protected animals. We found that intranasal inoculation of a sublethal dose of S. pneumoniae serotype 1 conferred complete protection against a subsequent challenge with a lethal dose of the same strain. Sublethal infection elicited a strong IgM and IgG antibody response against the capsular polysaccharide, as assessed one week later, and an exacerbated influx of neutrophils into the lungs immediately after the lethal challenge. Genome-wide microarray-based transcriptional analysis of whole lungs showed 149 differentially expressed genes among which we found upregulation of Il17a, Ifng and several IL-17A- and IFN-γ-related genes in protected versus non-protected mice. Kinetics analysis showed higher expression levels of Il17a in protected animals at all time points whereas Ifng was upregulated early in the protected mice and later in the non-protected animals. Intracelluar cytokine staining demonstrated that CD4(+) T cells account for a great proportion of the IL-17A produced in the lungs of protected animals. Overall, these results showed that an upregulation of IL-17A- and a timely regulation of IFN-γ-related gene expression, together with development of a Th17 response, are relevant characteristics of the protective immunity against S. pneumoniae acute pneumonia.
اللغة: English
تدمد: 0171-2985
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8807f098a53b6fb5516021e138dbc594
https://www.hal.inserm.fr/inserm-00926614
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....8807f098a53b6fb5516021e138dbc594
قاعدة البيانات: OpenAIRE
الوصف
تدمد:01712985