A Comprehensive Genome Survey Provides Novel Insights into Bile Salt Hydrolase (BSH) in Lactobacillaceae
| العنوان: | A Comprehensive Genome Survey Provides Novel Insights into Bile Salt Hydrolase (BSH) in Lactobacillaceae |
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| المؤلفون: | Yunhai Yi, Yunyun Lv, Xuejing Lei, Lifeng Liang, Junwei Qian, Gengyun Zhang |
| المصدر: | Molecules; Volume 23; Issue 5; Pages: 1157 Molecules, Vol 23, Iss 5, p 1157 (2018) Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Sequence analysis, Pharmaceutical Science, Genomics, Computational biology, Genome, Article, Homology (biology), Amidohydrolases, Analytical Chemistry, lcsh:QD241-441, cholesterol-lowering probiotics, 03 medical and health sciences, 0302 clinical medicine, Bacterial Proteins, lcsh:Organic chemistry, Phylogenetics, Catalytic Domain, Drug Discovery, Amino Acid Sequence, Physical and Theoretical Chemistry, Peptide sequence, Phylogeny, biology, Phylogenetic tree, Organic Chemistry, Lactobacillaceae, bile salt hydrolase (BSH), biology.organism_classification, Enzyme Activation, 030104 developmental biology, genome survey, Chemistry (miscellaneous), 030220 oncology & carcinogenesis, Molecular Medicine, Genome, Bacterial, BSH classification |
| الوصف: | Bile salt hydrolase (BSH) is a well-known enzyme that has been commonly characterized in probiotic bacteria, as it has cholesterol-lowering effects. However, its molecular investigations are scarce. Here, we build a local database of BSH sequences from Lactobacillaceae (BSH–SDL), and phylogenetic analysis and homology searches were employed to elucidate their comparability and distinctiveness among species. Evolutionary study demonstrates that BSH sequences in BSH–SDL are divided into five groups, named BSH A, B, C, D and E here, which can be the genetic basis for BSH classification and nomenclature. Sequence analysis suggests the differences between BSH-active and BSH-inactive proteins clearly, especially on site 82. In addition, a total of 551 BSHs from 107 species are identified from 451 genomes of 158 Lactobacillaceae species. Interestingly, those bacteria carrying various copies of BSH A or B can be predicted to be potential cholesterol-lowering probiotics, based on the results of phylogenetic analysis and the subtypes that those previously reported BSH-active probiotics possess. In summary, this study elaborates the molecular basis of BSH in Lactobacillaceae systematically, and provides a novel methodology as well as a consistent standard for the identification of the BSH subtype. We believe that high-throughput screening can be efficiently applied to the selection of promising candidate BSH-active probiotics, which will advance the development of healthcare products in cholesterol metabolism. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1420-3049 |
| DOI: | 10.3390/molecules23051157 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::880de7ede0f62e552c3b3c50281853b7 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....880de7ede0f62e552c3b3c50281853b7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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| DOI: | 10.3390/molecules23051157 |