Spinophilin is required for normal morphology, Ca2+ homeostasis and contraction but dispensable for β-adrenergic stimulation of adult cardiomyocytes
| العنوان: | Spinophilin is required for normal morphology, Ca2+ homeostasis and contraction but dispensable for β-adrenergic stimulation of adult cardiomyocytes |
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| المؤلفون: | Volkmar Gross, Daria Petzhold, André C. da Costa-Goncalves, Ingo Morano |
| المصدر: | Journal of Muscle Research and Cell Motility. 32:243-248 |
| بيانات النشر: | Springer Science and Business Media LLC, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Male, Scaffold protein, medicine.medical_specialty, Systole, Physiology, Adrenergic, Nerve Tissue Proteins, Stimulation, Biology, Biochemistry, Membrane Potentials, Mice, Norepinephrine, GTP-Binding Proteins, Internal medicine, Receptors, Adrenergic, beta, medicine, Animals, Homeostasis, Myocyte, Myocytes, Cardiac, Receptor, Cell Size, Mice, Knockout, Ryanodine receptor, Microfilament Proteins, Protein phosphatase 1, Cell Biology, Cell biology, Mice, Inbred C57BL, Endocrinology, Models, Animal, Calcium, Protein Binding, Signal Transduction |
| الوصف: | Spinophilin (SPN) is a ubiquitously expressed scaffolding protein that interacts through several binding modules with a variety of target proteins. Thus, SPN bundles F-actin, targets protein phosphatase 1 to the ryanodine receptor, and targets regulators of G-protein signaling to G-protein coupled receptors in cardiomyocytes. In this work we studied the role of SPN on cardiomyocyte morphology, function, and β-adrenergic responsiveness using a homozygous SPN knock-out mouse model (SPN-/-). We show that spinophilin deficiency significantly (1) reduced cardiomyocyte length, (2) increases both Ca(2+) amplitude and maximal rate of Ca(2+) rise during systole, and (3) decreased shortening amplitude and maximal rate of shortening, while (4) β-adrenergic stimulation remained intact. Our data suggest that spinophilin is an upstream regulator required for normal growth and excitation-contraction coupling, but is dispensable for β-adrenergic stimulation of adult cardiomyocytes. |
| تدمد: | 1573-2657 0142-4319 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8864a7b30e50440c2d3a8632b7932e0b https://doi.org/10.1007/s10974-011-9259-4 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....8864a7b30e50440c2d3a8632b7932e0b |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15732657 01424319 |
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