Investigations of immunogenic, allergenic and adjuvant properties of Cry1Ab protein after intragastric exposure in a food allergy model in mice

التفاصيل البيبلوغرافية
العنوان: Investigations of immunogenic, allergenic and adjuvant properties of Cry1Ab protein after intragastric exposure in a food allergy model in mice
المؤلفون: Terje Traavik, Odd Gunnar Wikmark, Unni Cecilie Nygaard, Thomas Bøhn, Martinus Løvik, Johanna Bodin, Monica Andreassen
المصدر: BMC Immunology
سنة النشر: 2016
مصطلحات موضوعية: 0301 basic medicine, Allergy, medicine.medical_treatment, Bacterial Toxins, Food, Genetically Modified, Immunology, Immunoglobulin E, Zea mays, Mice, 03 medical and health sciences, Biosafety, 0302 clinical medicine, Food allergy, medicine, Animals, Insect Proteins, Trypsin, Cry1Ab, Anaphylaxis, Mice, Inbred BALB C, Mice, Inbred C3H, Organisms, Genetically Modified, biology, Plant Extracts, fungi, food and beverages, Allergens, medicine.disease, Lupinus, Cryptochromes, Intestines, 030104 developmental biology, VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk immunologi: 716, 030220 oncology & carcinogenesis, Proteolysis, Genetically modified maize, biology.protein, Female, Edible Grain, Adjuvant, Food Hypersensitivity, Research Article
الوصف: Background: In genetically modified (GM) crops there is a risk that the inserted genes may introduce new allergens and/or adjuvants into the food and feed chain. The MON810 maize, expressing the insecticidal Cry1Ab toxin, is grown in many countries worldwide. In animal models, intranasal and intraperitoneal immunisations with the purified Cry1Ab proteins have induced immune responses, and feeding trials with Cry1Ab-containing feed have revealed some altered immune responses. Previous investigations have primarily measured antibody responses to the protein, while investigations of clinical food allergy symptoms, or allergy promotion (adjuvant effect) associated with the Cry1Ab protein are largely missing. We aimed to investigate immunogenic, allergenic and adjuvant properties of purified Cry1Ab toxin (trypCry1Ab, i.e., trypsin activated Cry1Ab) in a mouse model of food allergy. Method: Female C3H/HeJ mice were immunized by intragastric gavage of 10 μg purified, trypsin activated Cry1Ab toxin (trypCry1Ab) alone or together with the food allergen lupin. Cholera toxin was added as a positive control for adjuvant effect to break oral tolerance. Clinical symptoms (anaphylaxis) as well as humoral and cellular responses were assessed. Results: In contrast to results from previous airway investigations, we observed no indication of immunogenic properties of trypCry1Ab protein after repeated intragastric exposures to one dose, with or without CT as adjuvant. Moreover, the results indicated that trypCry1Ab given by the intragastric route was not able to promote allergic responses or anaphylactic reactions against the co-administered allergen lupin at the given dose. Conclusion: The study suggests no immunogenic, allergenic or adjuvant capacity of the given dose of trypCry1Ab protein after intragastric exposure of prime aged mice. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://​creativecommons.​org/​licenses/​by/​4.​0/​), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://​creativecommons.​org/​publicdomain/​zero/​1.​0/​) applies to the data made available in this article, unless otherwise stated
وصف الملف: application/pdf
اللغة: English
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::887ce2f21f614dca9150b01ec123ff41
http://hdl.handle.net/11250/2393618
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....887ce2f21f614dca9150b01ec123ff41
قاعدة البيانات: OpenAIRE