Inhibition of Sox2 Expression in the Adult Neural Stem Cell Niche In Vivo by Monocationic-based siRNA Delivery
| العنوان: | Inhibition of Sox2 Expression in the Adult Neural Stem Cell Niche In Vivo by Monocationic-based siRNA Delivery |
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| المؤلفون: | Patrick Neuberg, Patrick Erbacher, Marie-Elise Bonnet, Rym Ghaddab, Ghislaine Morvan-Dubois, Silvia Alejandra López-Juárez, Fabrice Stock, Anne-Laure Bolcato-Bellemin, Jacqueline Pierre-Simons, Barbara A. Demeneix, Sylvie Remaud, Marie Stéphanie Clerget-Froidevaux |
| المصدر: | Molecular Therapy: Nucleic Acids, Vol 2, Iss C (2013) Molecular Therapy. Nucleic Acids Molecular Therapy — Nucleic Acids; Vol 2 |
| بيانات النشر: | Elsevier, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Sox2, adult neural stem cells, Subventricular zone, non-viral transfection, Biology, Stem cell marker, monocationic lipid vector, 03 medical and health sciences, 0302 clinical medicine, SOX2, RNA interference, Drug Discovery, medicine, Progenitor cell, 030304 developmental biology, 0303 health sciences, Gene knockdown, lcsh:RM1-950, Neural stem cell, Cell biology, in vivo, medicine.anatomical_structure, lcsh:Therapeutics. Pharmacology, nervous system, 030220 oncology & carcinogenesis, siRNA, Immunology, Molecular Medicine, Original Article, Stem cell |
| الوصف: | RNA interference (RNAi) is a major tool for basic and applied investigations. However, obtaining RNAi data that have physiological significance requires investigation of regulations and therapeutic strategies in appropriate in vivo settings. To examine in vivo gene regulation and protein function in the adult neural stem cell (NSC) niche, we optimized a new non-viral vector for delivery of siRNA into the subventricular zone (SVZ). This brain region contains the neural stem and progenitor cells populations that express the stem cell marker, SOX2. Temporally and spatially controlled Sox2 knockdown was achieved using the monocationic lipid vector, IC10. siRNA/IC10 complexes were stable over time and smaller (40 nm) than jetSi complexes (≈400 nm). Immunocytochemistry showed that siRNA/IC10 complexes efficiently target both the progenitor and stem cell populations in the adult SVZ. Injection of the complexes into the lateral brain ventricle resulted in specific knockdown of Sox2 in the SVZ. Furthermore, IC10-mediated transient in vivo knockdown of Sox2-modulated expression of several genes implicated in NSC maintenance. Taken together, these data show that IC10 cationic lipid formulation can efficiently vectorize siRNA in a specific area of the adult mouse brain, achieving spatially and temporally defined loss of function.Molecular Therapy-Nucleic Acids (2013) 2, e89; doi:10.1038/mtna.2013.8; published online 23 April 2013. |
| اللغة: | English |
| تدمد: | 2162-2531 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88e41ddc83627806b4dd9322d9f7acf6 http://www.sciencedirect.com/science/article/pii/S2162253116301482 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....88e41ddc83627806b4dd9322d9f7acf6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21622531 |
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