The Spatiotemporal Program of Replication in the Genome of Lachancea kluyveri
| العنوان: | The Spatiotemporal Program of Replication in the Genome of Lachancea kluyveri |
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| المؤلفون: | Nicolas Agier, Orso Maria Romano, Fabrice Touzain, Gilles Fischer, Marco Cosentino Lagomarsino |
| المساهمون: | Centre de Génétique Moléculaire, UPR 2167 - DNA MicroArray (GODMAP), Centre National de la Recherche Scientifique (CNRS), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Biologie Computationnelle et Quantitative = Laboratory of Computational and Quantitative Biology (LCQB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Génomique des Microorganismes (LGM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Agence Nationale pour la Recherche [BLAN1606], ATIP grant from the Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Genome Biology and Evolution Genome Biology and Evolution, Society for Molecular Biology and Evolution, 2013, 5 (2), pp.370-388. ⟨10.1093/gbe/evt014⟩ Genome Biology and Evolution, 2013, 5 (2), pp.370-388. ⟨10.1093/gbe/evt014⟩ |
| بيانات النشر: | HAL CCSD, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | DNA Replication, replication, Lachancea kluyveri, [SDV]Life Sciences [q-bio], Centromere, Replication Origin, Saccharomyces cerevisiae, Origin of replication, Pre-replication complex, Chromosomes, S Phase, 03 medical and health sciences, 0302 clinical medicine, Control of chromosome duplication, Genetics, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, GC skew, 0303 health sciences, Replication timing, GC content, biology, Chromosomal fragile site, DNA replication, Telomere, biology.organism_classification, ACS, GC Rich Sequence, Origin recognition complex, Genome, Fungal, 030217 neurology & neurosurgery, Research Article |
| الوصف: | International audience; We generated a genome-wide replication profile in the genome of Lachancea kluyveri and assessed the relationship between replication and base composition. This species diverged from Saccharomyces cerevisiae before the ancestral whole genome duplication. The genome comprises eight chromosomes among which achromosomal arm of 1Mb has a G + C-content much higher than the rest of the genome. We identified 252 active replication origins in L. kluyveri and found considerable divergence in origin location with S. cerevisiae and with Lachancea waltii. Although some global features of S. cerevisiae replication are conserved: Centromeres replicate early, whereas telomeres replicate late, we found that replication origins both in L. kluyveri and L. waltii do not behave as evolutionary fragile sites. In L. kluyveri, replication timing along chromosomes alternates between regions of early and late activating origins, except for the 1Mb GC-rich chromosomal arm. This chromosomal arm contains an origin consensus motif different from other chromosomes and is replicated early during S-phase. We showed that precocious replication results from the specific absence of late firing origins in this chromosomal arm. In addition, we found a correlation between GC-content and distance from replication origins as well as a lack of replication-associated compositional skew between leading and lagging strands specifically in this GC-rich chromosomal arm. These findings suggest that the unusual base composition in the genome of L. kluyveri could be linked to replication. |
| اللغة: | English |
| تدمد: | 1759-6653 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88f15efdea9142622cfaa0f05bc0fb5d https://hal.archives-ouvertes.fr/hal-01528417 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....88f15efdea9142622cfaa0f05bc0fb5d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17596653 |
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