MAPK-mediated auxin signal transduction pathways regulate the malic acid secretion under aluminum stress in wheat (Triticum aestivum L.)
| العنوان: | MAPK-mediated auxin signal transduction pathways regulate the malic acid secretion under aluminum stress in wheat (Triticum aestivum L.) |
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| المؤلفون: | Zhuqing Zhao, Xinwei Liu, Ye Yang, Xiuming Cui, Ying Liu, Ya-meng Lin, Chengxiao Wang, Diqiu Liu |
| المصدر: | Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) Scientific Reports |
| بيانات النشر: | Nature Portfolio, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, MAPK/ERK pathway, Science, Malates, Plant Roots, 01 natural sciences, Arsenicals, Article, 03 medical and health sciences, chemistry.chemical_compound, Downregulation and upregulation, Gene Expression Regulation, Plant, Stress, Physiological, heterocyclic compounds, Protein Kinase Inhibitors, Triticum, Plant Proteins, Malic acid secretion, Regulation of gene expression, Multidisciplinary, Indoleacetic Acids, biology, food and beverages, Cell biology, 030104 developmental biology, chemistry, Biochemistry, RNA, Plant, Isotope Labeling, Mitogen-activated protein kinase, biology.protein, Medicine, Malic acid, Efflux, Mitogen-Activated Protein Kinases, Signal transduction, Aluminum, Signal Transduction, 010606 plant biology & botany |
| الوصف: | An isobaric tags for relative and absolute quantitative (iTRAQ)-based quantitative proteomic approach was used to screen the differentially expressed proteins during control treatment (CK), aluminum (Al) and Al+ indole-3-acetic acid (IAA) treatment of wheat lines ET8 (Al-tolerant). Further, the the expression levels of auxin response factor (ARF), Aux/IAA, Mitogen activated protein kinase (MAPK) 2c, and MAPK1a were analyzed. Results showed that 16 proteins were determined to be differentially expressed in response to Al and IAA co-treatment compared with Al alone. Among them, MAPK2c and MAPK1a proteins displayed markedly differential expression during the processes. The expression of ARF2 was upregulated and Aux/IAA was downregulated by Al, while both in concentration- and time-dependent manners. Western-blot detection of MAPK2c and MAPK1a indicated that Al upregulated MAPK2c and downregulated MAPK1a in both concentration- and time-dependent manners. Exogenous IAA could promote the expression of MAPK2c, but inhibit the expression of MAPK1a in the presence/absence of Al. These findings indicated that IAA acted as one of the key signaling molecule controls the response mechanism of wheat malic acid efflux to Al stress through the suppression/activation of Aux/IAA and ARFs, and the activity of MAPK2c and MAPK1a were positively or negatively regulated. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::891180fc3295036e5b51e516bdd21db6 https://doaj.org/article/f8c8c9141e05460abc9fb54e11735101 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....891180fc3295036e5b51e516bdd21db6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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