The effect of neuropeptide FF in the amygdala kindling model
| العنوان: | The effect of neuropeptide FF in the amygdala kindling model |
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| المؤلفون: | Ine Buffel, Paul Boon, Wytse J. Wadman, Jean-Jacques Bourguignon, Frédéric Bihel, Kristl Vonck, Martine Schmitt, Frédéric Simonin, V. De Meulenaere, Lindsay Poppe, V. De Herdt, Jeanelle Portelli, Alfred Meurs, Ilse Julia Smolders, Robrecht Raedt |
| المساهمون: | Neuroprotection & Neuromodulation, Pharmaceutical Chemistry, Drug Analysis and Drug Information, Experimental Pharmacology, Laboratoire d'Innovation Thérapeutique (LIT), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC), RTM Alpes-Maritimes, ONF, Institut Gilbert-Laustriat : Biomolécules, Biotechnologie, Innovation Thérapeutique, Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Acta Neurologica Scandinavica Supplementum Acta Neurologica Scandinavica Supplementum, Wiley-Blackwell, 2016, 134 (3), pp.181-188. ⟨10.1111/ane.12526⟩ Europe PubMed Central |
| بيانات النشر: | Wiley-Blackwell, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, medicine.medical_specialty, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Central nervous system, Aucun, Stimulation, administration & dosage, pharmacology, Status epilepticus, Amygdala, 03 medical and health sciences, 0302 clinical medicine, Seizures, Internal medicine, Kindling, Neurologic, medicine, Animals, [CHIM]Chemical Sciences, Neuropeptide FF, Rats, Wistar, Saline, ComputingMilieux_MISCELLANEOUS, Chemistry, Kindling, Sciences du Vivant [q-bio]/Biotechnologies, General Medicine, Rats, drug therapy, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Anticonvulsant, Epilepsy, Temporal Lobe, Neurology, drug effects, Anticonvulsants, Neurology (clinical), medicine.symptom, Oligopeptides, 030217 neurology & neurosurgery |
| الوصف: | Objective Neuropeptide FF (NPFF) and its receptors (NPFF1R and NPFF2R) are differentially distributed throughout the central nervous system. NPFF reduces cortical excitability in rats when administered intracerebroventricularly (i.c.v.), and both NPFF and NPFF1R antagonists attenuate pilocarpine-induced limbic seizures. In this study, our aim was to determine whether NPFF exerts anticonvulsant or anti-epileptogenic effects in the rat amygdala kindling model for temporal lobe seizures. Methods Male Wistar rats were implanted with a recording/stimulation electrode in the right amygdala and a cannula in the left lateral ventricle. In a first group of animals, the afterdischarge threshold (ADT) was determined after a single i.c.v. infusion of saline (n = 8) or NPFF (1 nmol/h for 2 h; n = 10). Subsequently, daily infusion of saline (n = 8) or NPFF (1 nmol/h for 2 h; i.c.v.; n = 9) was performed, followed by a kindling stimulus (ADT+200 μA). Afterdischarge duration and seizure severity were evaluated after every kindling stimulus. A second group of rats (n = 7) were fully kindled, and the effect of saline or a high dose of NPFF (10 nmol/h for 2 h, i.c.v.) on ADT and the generalized seizure threshold (GST) was subsequently determined. Results In naive rats, NPFF significantly increased the ADT compared to control (435 ± 72 μA vs 131 ± 23 μA [P < 0.05]). When rats underwent daily stimulations above the ADT, NPFF did not delay or prevent kindling acquisition. Furthermore, a high dose of NPFF did not alter ADT or GST in fully kindled rats. Conclusions I.c.v. administration of NPFF reduced excitability in the amygdala in naive, but not in fully kindled rats, and had no effect on kindling acquisition. |
| اللغة: | English |
| تدمد: | 0065-1427 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8925145dc1615b8016ab1568de2652d6 https://hdl.handle.net/20.500.14017/dde7ac56-bca6-463e-ad5c-55f134793215 |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....8925145dc1615b8016ab1568de2652d6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00651427 |
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