Scaffold-free human mesenchymal stem cell construct geometry regulates long bone regeneration
| العنوان: | Scaffold-free human mesenchymal stem cell construct geometry regulates long bone regeneration |
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| المؤلفون: | Honghyun Park, Yuxuan Cheng, Joel D. Boerckel, Samuel Herberg, Jung-Youn Shin, Eben Alsberg, Daniel Varghai, Phuong N. Dang, Anna D. Dikina, Daniel S. Alt, Marsha W. Rolle |
| المصدر: | Communications Biology Communications Biology, Vol 4, Iss 1, Pp 1-16 (2021) |
| بيانات النشر: | University of Illinois at Chicago, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Scaffold, Bone Regeneration, QH301-705.5, Long bone, Medicine (miscellaneous), Bone Morphogenetic Protein 2, Geometry, Biocompatible Materials, 02 engineering and technology, Bone morphogenetic protein, Bone morphogenetic protein 2, General Biochemistry, Genetics and Molecular Biology, Bone and Bones, Article, Transforming Growth Factor beta1, 03 medical and health sciences, Tissue engineering, Osteogenesis, medicine, Humans, Biology (General), Progenitor cell, Bone regeneration, Endochondral ossification, Cells, Cultured, 030304 developmental biology, Uncategorized, 0303 health sciences, Wound Healing, Tissue Engineering, Chemistry, Cartilage, Mesenchymal stem cell, Bone development, Cell Differentiation, Mesenchymal Stem Cells, 021001 nanoscience & nanotechnology, Chondrogenesis, equipment and supplies, Stem-cell research, 030104 developmental biology, medicine.anatomical_structure, Regenerative medicine, Collagen, General Agricultural and Biological Sciences, 0210 nano-technology, Morphogen |
| الوصف: | Biomimetic bone tissue engineering strategies partially recapitulate development. We recently showed functional restoration of femoral defects using scaffold-free human mesenchymal stem cell (hMSC) condensates featuring localized morphogen presentation with delayed in vivo mechanical loading. Possible effects of construct geometry on healing outcome remain unclear. Here, we hypothesized that localized presentation of transforming growth factor (TGF)-β1 and bone morphogenetic protein (BMP)-2 to engineered hMSC tubes mimicking femoral diaphyses induces endochondral ossification, and that TGF-β1 + BMP-2-presenting hMSC tubes enhance defect healing with delayed in vivo loading vs. loosely packed hMSC sheets. Localized morphogen presentation stimulated chondrogenic priming/endochondral differentiation in vitro. Subcutaneously, hMSC tubes formed cartilage templates that underwent bony remodeling. Orthotopically, hMSC tubes stimulated more robust endochondral defect healing vs. hMSC sheets. Tissue resembling normal growth plate was observed with negligible ectopic bone. This study demonstrates interactions between hMSC condensation geometry, morphogen bioavailability, and mechanical cues to recapitulate development for biomimetic bone tissue engineering. Herberg et al. previously showed functional healing of femoral defects using scaffold-free human mesenchymal stem cell (hMSC) condensates with localized morphogen presentation. In this study, they report the importance of the tubular geometry of MSC condensates in long bone regeneration. Unlike loosely packed hMSC sheets, only hMSC tubes induced regenerate tissue partially resembling normal growth plate. |
| DOI: | 10.25417/uic.14854407 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::898e3b4ec73a213e9fd38e45fe0c0494 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....898e3b4ec73a213e9fd38e45fe0c0494 |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.25417/uic.14854407 |
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