Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against Helicobacter felis
| العنوان: | Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against Helicobacter felis |
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| المؤلفون: | Tao Xi, Menghui Fan, Wei Liu, Zhenxing Zhang, Ningyin Xu, Yingying Xing, An Huang, Guojing Ruan, Chupeng Hu, Yue Wang |
| المصدر: | Emerging Microbes & Infections article-version (VoR) Version of Record |
| بيانات النشر: | Taylor & Francis, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, CD4-Positive T-Lymphocytes, Receptors, CXCR3, Epidemiology, Neutrophils, 030106 microbiology, Immunology, Fibroin, CD8-Positive T-Lymphocytes, CXCR3, Microbiology, Gastric intraepithelial CD4+TRM cells, Helicobacter Infections, 03 medical and health sciences, Mice, Virology, Drug Discovery, medicine, CXCL10, Distribution (pharmacology), Animals, Pathogen, biology, Chemistry, digestive, oral, and skin physiology, Hydrogels, General Medicine, biology.organism_classification, Molecular biology, Epithelium, digestive system diseases, Vaccination, Chemokine CXCL10, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, silk fibroin hydrogel, Gastric Mucosa, Bacterial Vaccines, Helicobacter felis, Parasitology, Fibroins, Immunologic Memory, Research Article |
| الوصف: | Tissue-resident memory T (TRM) cells, located in the epithelium of most peripheral tissues, constitute the first-line defense against pathogen infections. Our previous study reported that gastric subserous layer (GSL) vaccination induced a “pool” of protective tissue-resident memory CD4+T (CD4+TRM) cells in the gastric epithelium. However, the mechanistic details how CD4+TRM cells form in the gastric epithelium are unknown. Here, our results suggested that the vaccine containing CCF in combination with Silk fibroin hydrogel (SF) broadened the distribution of gastric intraepithelial CD4+TRM cells. It was revealed that the gastric intraepithelial TRM cells were even more important than circulating memory T cells against infection by Helicobacter felis. It was also shown that gastric-infiltrating neutrophils were involved as indispensable mediators which secreted CXCL10 to chemoattract CXCR3+CD4+T cells into the gastric epithelium. Blocking of CXCR3 or neutrophils significantly decreased the number of gastric intraepithelial CD4+TRM cells due to reduced recruitment of CD4+T cells. This study demonstrated the protective efficacy of gastric CD4+TRM cells against H. felis infection, and highlighted the influence of neutrophils on gastric intraepithelial CD4+TRM cells formation. |
| اللغة: | English |
| تدمد: | 2222-1751 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89a5a9c1bafd9c8fdb29d81f767121bc http://europepmc.org/articles/PMC7594714 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....89a5a9c1bafd9c8fdb29d81f767121bc |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 22221751 |
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