Non-invasive in vivo optical imaging of the lacZ and luc gene expression in mice
| العنوان: | Non-invasive in vivo optical imaging of the lacZ and luc gene expression in mice |
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| المؤلفون: | Philippe Huber, Véronique Josserand, Isabelle Texier-Nogues, Marie Favrot, Jean-Luc Coll |
| المساهمون: | ANIMAGE, Rhône-Alpes Genopole, Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Physiopathologie vasculaire : interactions cellulaires, signalisation et vieillissement, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM U823, équipe 5 (cibles diagnostiques ou thérapeutiques et vectorisation de drogues dans le cancer du poumon), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Hurbin, Amandine |
| المصدر: | Gene Therapy Gene Therapy, 2007, 14 (22), pp.1587-93. ⟨10.1038/sj.gt.3303028⟩ Gene Therapy, Nature Publishing Group, 2007, 14 (22), pp.1587-93. ⟨10.1038/sj.gt.3303028⟩ |
| بيانات النشر: | HAL CCSD, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Luminescence, beta-Gal, Gene Expression, MESH: Microscopy, Fluorescence, MESH: Genetic Markers, Mice, 0302 clinical medicine, Genes, Reporter, Neoplasms, Gene expression, MESH: Animals, MESH: Neoplasms, Luciferases, 0303 health sciences, MESH: Luminescence, MESH: Lac Operon, reporter gene, MESH: beta-Galactosidase, Lac Operon, 030220 oncology & carcinogenesis, Molecular Medicine, MESH: Luminescent Proteins, fluorescence, Genetic Markers, Genetically modified mouse, MESH: Gene Expression, MESH: Mice, Transgenic, Mice, Transgenic, [SDV.CAN]Life Sciences [q-bio]/Cancer, Biology, transgenic mice, Transfection, 03 medical and health sciences, optical imaging, [SDV.CAN] Life Sciences [q-bio]/Cancer, In vivo, Genetics, Animals, Bioluminescence, Luciferase, Molecular Biology, Gene, MESH: Mice, 030304 developmental biology, Reporter gene, MESH: Transfection, MESH: Genes, Reporter, Genetic Therapy, beta-Galactosidase, Molecular biology, In vitro, Luminescent Proteins, Microscopy, Fluorescence, MESH: Luciferases, MESH: Gene Therapy |
| الوصف: | International audience; The bacterial lacZ gene encoding for beta-galactosidase (beta-gal) is a common reporter gene used in transgenic mice. Nonetheless, the absence of fluorigenic substrates usable in live animals greatly hampered the non-invasive follow-up of this reporter gene expression. We used far-red fluorescence for imaging beta-Gal expression in live cells in vitro or in vivo. The 9H-(1,3-dichloro-9,9-dimethylacridin- 2-one-7-yl) beta-D-galactopyranoside substrate was used to monitor beta-Gal expression as a reporter of tumor growth, or of the physiological levels of an endogenous gene or of gene transfer in lung. A quantitative evaluation of this method as well as a comparison of its sensitivity with Firefly Luciferase-based bioluminescence was also performed. In vivo measurements showed that 10(3) beta-Gal tumor cells located under the skin were detectable. In deeper organs like lung, as little as 5 ng of beta-Gal or Luciferase enzymes per mg of proteins were measured, confirming that both techniques reached similar sensibilities. Nonetheless, quantitative comparison of beta-Gal levels measured with far-red imaging or with a standardized enzymatic evaluation after killing revealed that the 2D-fluorescent reflectance imaging method is submitted to a color-dependent disparity of the organs and cannot supply quantitative measurements but that a simple correction can be applied. |
| اللغة: | English |
| تدمد: | 0969-7128 1476-5462 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a336128a5ceb3044d7b551b80237aec https://www.hal.inserm.fr/inserm-00313784 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8a336128a5ceb3044d7b551b80237aec |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09697128 14765462 |
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