Dissection and cytological mapping of chromosome arm 4VS by the development of wheat-Haynaldia villosa structural aberration library
العنوان: | Dissection and cytological mapping of chromosome arm 4VS by the development of wheat-Haynaldia villosa structural aberration library |
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المؤلفون: | Peidu Chen, Yuan Chunxia, Ruiqi Zhang, Xiue Wang, Zongkuan Wang, Yingbo Li, Haojie Sun, Haiyan Wang, Keli Dai, Renhui Zhao, Miaomiao Shi, Aizhong Cao, Qi Jia, Zhongyu Yu, Jin Xiao |
المصدر: | Theoretical and Applied Genetics. 133:217-226 |
بيانات النشر: | Springer Science and Business Media LLC, 2019. |
سنة النشر: | 2019 |
مصطلحات موضوعية: | Genetic Markers, 0106 biological sciences, Mutant, Chromosomal translocation, Genes, Plant, Dasypyrum villosum, 01 natural sciences, Chromosomes, Plant, Gene mapping, Mosaic Viruses, Sequence Homology, Nucleic Acid, Genetics, Aegilops tauschii, Triticum, Disease Resistance, Gene Library, Plant Diseases, Ions, Recombination, Genetic, biology, Chromosome Mapping, food and beverages, General Medicine, biology.organism_classification, Genetic Loci, Genetic marker, Chromosome Arm, Cytogenetic Analysis, Wheat yellow mosaic virus, Agronomy and Crop Science, 010606 plant biology & botany, Biotechnology |
الوصف: | A cytological map of Haynaldia villosa chromosome arm 4VS was constructed to facilitate the identification and utilization of beneficial genes on 4VS. Induction of wheat-alien chromosomal structure aberrations not only provides new germplasm for wheat improvement, but also allows assignment of favorable genes to define physical regions. Especially, the translocation or introgression lines carrying alien chromosomal fragments with different sizes are useful for breeding and alien gene mapping. Chromosome arm 4VS of Haynaldia villosa (L.) Schur (syn. Dasypyrum villosum (L.) P. Candargy) confers resistances to eyespot and wheat yellow mosaic virus (WYMV). In this research, we used both irradiation and the pairing homoeologous gene (Ph) mutant to induce chromosomal aberrations or translocations. By using the two approaches, a structural aberration library of chromosome arm 4VS was constructed. In this library, there are 57 homozygous structural aberrations, in which, 39 were induced by the Triticum aestivum cv. Chinese Spring (CS) ph1b mutant (CS ph1b) and 18 were induced by irradiation. The aberrations included four types, i.e., terminal translocation, interstitial translocation, deletion and complex structural aberration. The 4VS cytological map was constructed by amplification in the developed homozygous aberrations using 199 4VS-specific markers, which could be allocated into 39 bins on 4VS. These bins were further assigned to their corresponding physical regions of chromosome arm 4DS based on BLASTn search of the marker sequences against the reference sequence of Aegilops tauschii Cosson. The developed genetic stocks and cytological map provide genetic stocks for wheat breeding as well as alien gene tagging. |
تدمد: | 1432-2242 0040-5752 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a355121d65ae2becd26fe2f0ec9f3d2 https://doi.org/10.1007/s00122-019-03452-8 |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi.dedup.....8a355121d65ae2becd26fe2f0ec9f3d2 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 14322242 00405752 |
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