التفاصيل البيبلوغرافية
العنوان:
Three-dimensional imaging and uptake of the anticancer drug combretastatin in cell spheroids and photoisomerization in gels with multiphoton excitation
المؤلفون:
Stanley W. Botchway , Kathrin M. Scherer , John A. Hadfield , John W. Haycock , Anthony W. Parker , Roger H. Bisby
بيانات النشر:
Society of Photo-optical Instrumentation Engineers (SPIE), 2015.
سنة النشر:
2015
مصطلحات موضوعية:
Fluorescence-lifetime imaging microscopy , Materials science , Photoisomerization , Biomedical Engineering , Antineoplastic Agents , law.invention , Biomaterials , 03 medical and health sciences , chemistry.chemical_compound , Imaging, Three-Dimensional , 0302 clinical medicine , Optics , Confocal microscopy , law , Cell Line, Tumor , Spheroids, Cellular , Bibenzyls , Microscopy , Tumor Cells, Cultured , Humans , Fluorescent Dyes , 030304 developmental biology , Combretastatin , Photons , 0303 health sciences , business.industry , Spheroid , Fluorescence , Atomic and Molecular Physics, and Optics , Electronic, Optical and Magnetic Materials , Microscopy, Fluorescence, Multiphoton , chemistry , 030220 oncology & carcinogenesis , Femtosecond , Biophysics , business
الوصف:
The uptake of E-combretastatins, potential prodrugs of the anticancer Z-isomers, into multicellular spheroids has been imaged by intrinsic fluorescence in three dimensions using two-photon excited fluorescence lifetime imaging with 625-nm ultrafast femtosecond laser pulses. Uptake is initially observed at the spheroid periphery but extends to the spheroid core within 30 min. Using agarose gels as a three-dimensional model, the conversion of Z ðtransÞ → E ðcisÞ via two-photon photoisomerization is demonstrated and the location of this photochemical process may be precisely selected within the micron scale in all three dimensions at depths up to almost 2 mm. We discuss these results for enhanced tissue penetration at longer near-infrared wave- lengths for cancer therapy and up to three-photon excitation and imaging using 930-nm laser pulses with suitable combretastatin analogs. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or repro
وصف الملف:
application/pdf
اللغة:
English
تدمد:
1083-3668
URL الوصول:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a41867b24d2a55a8cccb76d673e95f2 https://usir.salford.ac.uk/id/eprint/35096/7/JBO_20_7_078003.pdf
حقوق:
OPEN
رقم الأكسشن:
edsair.doi.dedup.....8a41867b24d2a55a8cccb76d673e95f2
قاعدة البيانات:
OpenAIRE
