Carvedilol Ameliorates Intrahepatic Angiogenesis, Sinusoidal Remodeling and Portal Pressure in Cirrhotic Rats
| العنوان: | Carvedilol Ameliorates Intrahepatic Angiogenesis, Sinusoidal Remodeling and Portal Pressure in Cirrhotic Rats |
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| المؤلفون: | Liping Ling, Sining Wang, Dongxiao Meng, Guangqi Li, Chunqing Zhang |
| المصدر: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| بيانات النشر: | International Scientific Literature, Inc., 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Liver Cirrhosis, Male, Cirrhosis, Angiogenesis, Portal venous pressure, Pharmacology, Umbilical vein, 03 medical and health sciences, 0302 clinical medicine, In vivo, Hypertension, Portal, medicine, Human Umbilical Vein Endothelial Cells, Animals, Humans, Rats, Wistar, Carvedilol, Neovascularization, Pathologic, business.industry, Animal Study, Endothelial Cells, General Medicine, medicine.disease, Portal Pressure, Capillaries, Fibronectins, Rats, Liver, 030220 oncology & carcinogenesis, Portal hypertension, 030211 gastroenterology & hepatology, Angiogenesis Inducing Agents, business, medicine.drug, Transforming growth factor |
| الوصف: | BACKGROUND Carvedilol is the first-line drug for the primary prophylaxis of variceal bleeding due to portal hypertension (PHT) in liver cirrhosis. This study aimed to investigate the effects of carvedilol on intrahepatic angiogenesis and sinusoidal remodeling in cirrhotic rats and explore the underlying mechanisms of carvedilol in PHT. MATERIAL AND METHODS For in vivo experiments, carbon tetrachloride was used to induce liver cirrhosis in rats, and carvedilol was simultaneously administered by gavage. The portal pressure was measured in rats, and liver tissues were examined by immunohistochemistry. Sinusoidal remodeling was observed by transmission electron microscopy. For in vitro experiments, the effects of carvedilol on fibronectin (FN) synthesis in human umbilical vein endothelial cells (HUVECs) were explored by quantitative real-time polymerase chain reaction and western blot analysis. RESULTS Portal vein pressure measurements showed that carvedilol reduced portal pressure in cirrhotic rats. Immunohistochemistry assays indicated that carvedilol ameliorated intrahepatic angiogenesis. Transmission electron microscopy examination demonstrated that carvedilol improved sinusoidal remodeling. In the in vitro experiments, carvedilol suppressed transforming growth factor β1 (TGFβ1)-induced FN synthesis in HUVECs by inhibition of the TGFβ1/Smads pathway. CONCLUSIONS Carvedilol ameliorated intrahepatic angiogenesis, sinusoidal remodeling and portal pressure in cirrhotic rats. Carvedilol improved sinusoidal remodeling by suppressing FN synthesis in endothelial cells. Carvedilol has potential utility for treating early-stage liver cirrhosis. |
| اللغة: | English |
| تدمد: | 1643-3750 1234-1010 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a53b53581ca6e394b5c64bf7cb43561 http://europepmc.org/articles/PMC6253986 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8a53b53581ca6e394b5c64bf7cb43561 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16433750 12341010 |
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