Apoptotic Effects of Some Tetrahydronaphthalene Derivatives on K562 Human Chronic Myelogenous Leukemia Cell Line
| العنوان: | Apoptotic Effects of Some Tetrahydronaphthalene Derivatives on K562 Human Chronic Myelogenous Leukemia Cell Line |
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| المؤلفون: | Zeliha Büyükbingöl, Arzu Zeynep Karabay, Fugen Aktan, Ayse Selen Gurkan-Alp, Aslı Koç, Tulin Ozkan, Asuman Sunguroglu, Erdem Buyukbingol, Yalda Hekmatshoar |
| المصدر: | Anti-cancer agents in medicinal chemistry. 17(14) |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Tetrahydronaphthalenes, medicine.drug_class, Cell Survival, Retinoic acid, Caspase 3, Antineoplastic Agents, Apoptosis, 03 medical and health sciences, chemistry.chemical_compound, Structure-Activity Relationship, 0302 clinical medicine, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, medicine, Tumor Cells, Cultured, Humans, Viability assay, Retinoid, Cell Proliferation, Pharmacology, Dose-Response Relationship, Drug, Molecular Structure, Chemistry, medicine.disease, Leukemia, 030104 developmental biology, 030220 oncology & carcinogenesis, Cancer research, Molecular Medicine, Drug Screening Assays, Antitumor, K562 Cells, Chronic myelogenous leukemia, K562 cells |
| الوصف: | BACKGROUND Retinoids which are vitamin A (Retinol) derivatives have been suggested to mediate the inhibition of cancer cell growth and apoptosis. It has been reported that all trans retinoic acid (ATRA) exhibited suppressive effects on different types of leukemia including chronic myelogenous leukemia. OBJECTIVE In the present study, we aim to find out the effects of 6 synthetic N-(3,5,5,8,8-pentamethyl-5,6,7,8- tetrahydronaphthalene-2-yl)-carboxamide derivatives (compound 6-12) on cell viability and apoptotic pathways in K562 human chronic myelogenous leukemia cell line. METHODS Cell viability and apoptosis were examined by spectrophotometric thiazolyl blue tetrazolium bromide (MTT) and caspase-3 assay, western blot, RT-PCR and flow cytometry. RESULTS Our results indicated that compound 6 (5-(1,2-Dithiolan-3-yl)-N-(3,5,5,8,8-pentamethyl-5,6,7,8- tetrahydronaphthalen-2-yl)pentanamide), 8 (4-(3,4-Dimethoxyphenyl)-N-(3,5,5,8,8-pentamethyl-5,6,7,8- tetrahydronaphthalen-2-yl)butanamide) and 11 (E-3-(4-Hydroxy-3-methoxyphenyl)-N-(3,5,5,8,8-pentamethyl- 5,6,7,8-tetrahydronaphthalen-2-yl)acrylamide) exhibited apoptotic effects in K562 human chronic myelogenous leukemia cell line and induced caspase 3, PARP cleavage, Bax/Bcl-2 ratio, Bad and Bim gene expressions. CONCLUSION Some retinoid derivatives tested in this study induced apoptosis of K562 cells which suggest that these compounds may serve as potential agents in the treatment of chronic myelogenous leukemia. |
| تدمد: | 1875-5992 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a77956355f779b62e4029fa984b0283 https://pubmed.ncbi.nlm.nih.gov/28403785 |
| رقم الأكسشن: | edsair.doi.dedup.....8a77956355f779b62e4029fa984b0283 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18755992 |
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