PLK1- and PLK4-mediated asymmetric mitotic centrosome size and positioning in the early zebrafish embryo

التفاصيل البيبلوغرافية
العنوان: PLK1- and PLK4-mediated asymmetric mitotic centrosome size and positioning in the early zebrafish embryo
المؤلفون: Xiaofei Bai, Abrar A. Aljiboury, Lindsay Rathbun, Nicole A. Hall, Jeffrey D. Amack, Julie Manikas, Joshua N. Bembenek, Heidi Hehnly
المصدر: Curr Biol
سنة النشر: 2020
مصطلحات موضوعية: 0301 basic medicine, PLK4, animal structures, Embryo, Nonmammalian, Intravital Microscopy, Embryonic Development, Mitosis, Cell Cycle Proteins, Spindle Apparatus, Biology, Protein Serine-Threonine Kinases, PLK1, General Biochemistry, Genetics and Molecular Biology, Article, Animals, Genetically Modified, 03 medical and health sciences, 0302 clinical medicine, Microtubule, Proto-Oncogene Proteins, Animals, Caenorhabditis elegans, Zebrafish, Cell Size, Centrosome, Microscopy, Confocal, Zebrafish Proteins, biology.organism_classification, Embryonic stem cell, Cell biology, Spindle apparatus, 030104 developmental biology, embryonic structures, General Agricultural and Biological Sciences, 030217 neurology & neurosurgery
الوصف: Summary Factors that regulate mitotic spindle positioning remain unclear within the confines of extremely large embryonic cells, such as the early divisions of the vertebrate embryo, Danio rerio (zebrafish). We find that the mitotic centrosome, a structure that assembles the mitotic spindle [ 1 ], is notably large in the zebrafish embryo (246.44 ± 11.93 μm2 in a 126.86 ± 0.35 μm diameter cell) compared to a C. elegans embryo (5.78 ± 0.18 μm2 in a 55.83 ± 1.04 μm diameter cell). During embryonic cell divisions, cell size changes rapidly in both C. elegans and zebrafish [ 2 , 3 ], where mitotic centrosome area scales more closely with changes in cell size compared to changes in spindle length. Embryonic zebrafish spindles contain asymmetrically sized mitotic centrosomes (2.14 ± 0.13-fold difference between the two), with the larger mitotic centrosome placed toward the embryo center in a polo-like kinase (PLK) 1- and PLK4-dependent manner. We propose a model in which uniquely large zebrafish embryonic centrosomes direct spindle placement within disproportionately large cells.
اللغة: English
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8adaf7ddc630bc7ecd0449d58b569aa1
https://europepmc.org/articles/PMC8159022/
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....8adaf7ddc630bc7ecd0449d58b569aa1
قاعدة البيانات: OpenAIRE