Mir-573 regulates cell proliferation and apoptosis by targeting Bax in human degenerative disc cells following hyperbaric oxygen treatment
| العنوان: | Mir-573 regulates cell proliferation and apoptosis by targeting Bax in human degenerative disc cells following hyperbaric oxygen treatment |
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| المؤلفون: | Kuo-Chen Wei, Song-Shu Lin, Chi-Chien Niu, Meng-Ling Lu, Chiung-Yin Huang, Chuen-Yung Yang, Kowit-Yu Chong, Li-Jen Yuan, Steve W. N. Ueng, Tsung-Ting Tsai, Po-Liang Lai |
| المصدر: | Journal of Orthopaedic Surgery and Research, Vol 16, Iss 1, Pp 1-10 (2021) Journal of Orthopaedic Surgery and Research |
| بيانات النشر: | Research Square Platform LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Nucleus Pulposus, lcsh:Diseases of the musculoskeletal system, Gene Expression, HBO, Apoptosis, Caspase 3, Intervertebral Disc Degeneration, NP, 03 medical and health sciences, 0302 clinical medicine, lcsh:Orthopedic surgery, microRNA, Humans, Medicine, Orthopedics and Sports Medicine, Cells, Cultured, Caspase, Aged, Cell Proliferation, bcl-2-Associated X Protein, Caspase-9, Hyperbaric Oxygenation, Gene knockdown, biology, Cell growth, business.industry, Transfection, Middle Aged, Molecular biology, MicroRNAs, lcsh:RD701-811, 030104 developmental biology, Bax, 030220 oncology & carcinogenesis, biology.protein, Female, Surgery, lcsh:RC925-935, business, MicroRNA-573, Research Article |
| الوصف: | Background MicroRNA (miRNA) plays a vital role in the intervertebral disc (IVD) degeneration. The expression level of miR-573 was downregulated whereas Bax was upregulated notably in human degenerative nucleus pulposus cells. In this study, we aimed to investigate the role of miR-573 in human degenerative nucleus pulposus (NP) cells following hyperbaric oxygen (HBO) treatment. Methods NP cells were separated from human degenerated IVD tissues. The control cells were maintained in 5% CO2/95% air and the hyperoxic cells were exposed to 100% O2 at 2.5 atmospheres absolute. MiRNA expression profiling was performed via microarray and confirmed by real-time PCR, and miRNA target genes were identified using bioinformatics and luciferase reporter assays. The mRNA and protein levels of Bax were measured. The proliferation of NPCs was detected using MTT assay. The protein expression levels of Bax, cleaved caspase 9, cleaved caspase 3, pro-caspase 9, and pro-caspase 3 were examined. Results Bioinformatics analysis indicated that the 3′ untranslated region (UTR) of the Bax mRNA contained the “seed-matched-sequence” for hsa-miR-573, which was validated via reporter assays. MiR-573 was induced by HBO and simultaneous suppression of Bax was observed in NP cells. Knockdown of miR-573 resulted in upregulation of Bax expression in HBO-treated cells. In addition, overexpression of miR-573 by HBO increased cell proliferation and coupled with inhibition of cell apoptosis. The cleavage of pro-caspase 9 and pro-caspase 3 was suppressed while the levels of cleaved caspase 9 and caspase 3 were decreased in HBO-treated cells. Transfection with anti-miR-573 partly suppressed the effects of HBO. Conclusion Mir-573 regulates cell proliferation and apoptosis by targeting Bax in human degenerative NP cells following HBO treatment. |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b0849bd47ca741d2957c612a7308a5a https://doi.org/10.21203/rs.3.rs-88633/v1 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8b0849bd47ca741d2957c612a7308a5a |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |