Role of seipin in lipid droplet morphology and hepatitis C virus life cycle
| العنوان: | Role of seipin in lipid droplet morphology and hepatitis C virus life cycle |
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| المؤلفون: | Catherine Fauvelle, Stéphanie Conzelmann, Kaori Minehira, Emilie Branche, V. Kaddai, Francesco Negro, Sophie Clément, Tujana Boldanova, Birke Bartosch |
| المصدر: | Journal of General Virology, Vol. 94, No Pt 10 (2013) pp. 2208-14 |
| بيانات النشر: | Microbiology Society, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Hepatitis C virus, Virus Replication/physiology, Neutral fat, Hepacivirus, Lipids/chemistry, ddc:616.07, Biology, Virus Replication, medicine.disease_cause, Seipin, chemistry.chemical_compound, Hepacivirus/physiology, GTP-Binding Protein gamma Subunits, Cell Line, Tumor, Virology, Lipid droplet, Organelle, medicine, Humans, ddc:616, Triglyceride, Gene Expression Regulation/physiology, Cholesterol, Virus Internalization, Lipids, Gene Expression Regulation, Biochemistry, chemistry, Cytoplasm, Biophysics, lipids (amino acids, peptides, and proteins), GTP-Binding Protein gamma Subunits/genetics/metabolism |
| الوصف: | Infectious hepatitis C virus (HCV) particle assembly starts at the surface of lipid droplets, cytoplasmic organelles responsible for neutral fat storage. We analysed the relationship between HCV and seipin, a protein involved in lipid droplet maturation. Although seipin overexpression did not affect the total mean volume occupied by lipid droplets nor the total triglyceride and cholesterol ester levels per cell, it caused an increase in the mean diameter of lipid droplets by 60 %, while decreasing their total number per cell. The latter two effects combined resulted in a 34 % reduction of the total outer surface area of lipid droplets per cell, with a proportional decrease in infectious viral particle production, probably due to a defect in particle assembly. These results suggest that the available outer surface of lipid droplets is a critical factor for HCV release, independent of the neutral lipid content of the cell. |
| تدمد: | 1465-2099 0022-1317 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bb3ad158772d5ea8fb6c485cc658371 https://doi.org/10.1099/vir.0.054593-0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8bb3ad158772d5ea8fb6c485cc658371 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652099 00221317 |
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