Glycine-extended gastrin activates two independent tyrosine-kinases in upstream of p85/p110 phosphatidylinositol 3-kinase in human colonic tumour cells
| العنوان: | Glycine-extended gastrin activates two independent tyrosine-kinases in upstream of p85/p110 phosphatidylinositol 3-kinase in human colonic tumour cells |
|---|---|
| المؤلفون: | Aline Kowalski-Chauvel, Julie Pannequin, Audrey Ferrand, Claudine Bertrand, Daniel Fourmy, Catherine Seva, Marlène Dufresne |
| المساهمون: | Biologie et Pathologie Digestive, IFR 31 Louis Bugnard (IFR 31), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Melbourne, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Herrada, Anthony |
| المصدر: | World Journal of Gastroenterology World Journal of Gastroenterology, Baishideng Publishing Group Co. Limited, 2006, 12 (12), pp.1859-1864. ⟨10.3748/wjg.v12.i12.1859⟩ World Journal of Gastroenterology, 2006, 12 (12), pp.1859-1864. ⟨10.3748/wjg.v12.i12.1859⟩ |
| بيانات النشر: | HAL CCSD, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | MESH: Signal Transduction, MESH: Gastrins, MESH: Janus Kinase 2, chemistry.chemical_compound, Phosphatidylinositol 3-Kinases, 0302 clinical medicine, Gastrin, 0303 health sciences, Janus kinase 2, biology, Kinase, Gastroenterology, General Medicine, Protein-Tyrosine Kinases, 3. Good health, Colon cancer, JAK2, 030220 oncology & carcinogenesis, embryonic structures, Colonic Neoplasms, Glycine-extended gastrin, Electrophoresis, Polyacrylamide Gel, Signal transduction, Tyrosine kinase, hormones, hormone substitutes, and hormone antagonists, Signal Transduction, Src, MESH: Enzyme Activation, animal structures, MESH: HCT116 Cells, Proto-Oncogene Proteins pp60(c-src), [SDV.CAN]Life Sciences [q-bio]/Cancer, MESH: Protein-Tyrosine Kinases, 03 medical and health sciences, Enzyme activator, [SDV.CAN] Life Sciences [q-bio]/Cancer, Proto-Oncogene Proteins, MESH: Cell Proliferation, Gastrins, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Humans, Immunoprecipitation, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Phosphatidylinositol, 030304 developmental biology, Cell Proliferation, Colorectal Cancer, MESH: Colonic Neoplasms, MESH: Humans, MESH: Immunoprecipitation, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, MESH: Proto-Oncogene Proteins pp60(c-src), Janus Kinase 2, HCT116 Cells, Molecular biology, [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Enzyme Activation, MESH: Proto-Oncogene Proteins, chemistry, MESH: Phosphatidylinositol 3-Kinases, biology.protein, Phosphatidylinositol 3-kinase, MESH: Electrophoresis, Polyacrylamide Gel |
| الوصف: | To investigate whether Src, JAK2 and phosphatidylinositol 3-kinase (PI3K) pathways are involved in the proliferation of human colonic tumour cells induced by glycine-extended gastrin (G-gly), the precursor of the mature amidated gastrin and to elucidate the molecular interaction between these three kinases in response to this peptide.Using the human colonic tumour cell line HCT116 as a model, we first measured the activation of PI3K, p60-Src and JAK2 in response to G-gly by in vitro kinase assays. Then we investigated the involvement of these kinases in G-gly-induced cell proliferation by MTT test.G-gly stimulation induced p60-Src, JAK2 and PI3K activation in HCT116. The different pathways were involved in proliferation of human colon cancer cells induced by G-gly. Furthermore, we found that both Src and JAK2 were necessary to PI3K regulation by this peptide. However, we did not find any cross-talk between the two tyrosine kinases.Our results suggest that the p60-Src/PI3K and JAK2/PI3K pathways act independently to mediate G-gly proliferative effect on human colonic tumour cells. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1007-9327 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bc9da0ce77f044d46e071aa7d54416c https://hal.archives-ouvertes.fr/hal-03108771 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8bc9da0ce77f044d46e071aa7d54416c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10079327 |
|---|