Liposome destruction by hydrodynamic cavitation in comparison to chemical, physical and mechanical treatments
| العنوان: | Liposome destruction by hydrodynamic cavitation in comparison to chemical, physical and mechanical treatments |
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| المؤلفون: | Žiga Pandur, Matevž Dular, Iztok Dogsa, David Stopar |
| المصدر: | Ultrasonics Sonochemistry Ultrasonics Sonochemistry, vol. 61, no. 104826, 2020. |
| بيانات النشر: | Elsevier BV, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | fluorescein, Acoustics and Ultrasonics, kemijski postopki, confocal laser microscopy, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, lipidni vezikli, Inorganic Chemistry, Surface-Active Agents, chemistry.chemical_compound, hydrodynamic cavitation, fluorescein release, Chemical Engineering (miscellaneous), Environmental Chemistry, giant lipid vesicles, Radiology, Nuclear Medicine and imaging, Centrifugation, Fluorescein, Liposome, udc:577.11:576.3:547.915:620.193.16, Sonotrode, Chemistry, business.industry, Vesicle, Organic Chemistry, Ultrasound, mehanski postopki, stability, modelne membrane, 021001 nanoscience & nanotechnology, Lipids, 0104 chemical sciences, Microscopy, Fluorescence, Ionic strength, DOPC, fluorescenčna mikroskopija, Cavitation, Liposomes, Hydrodynamics, Biophysics, fizikalni postopki, hidrodinamska kavitacija, 0210 nano-technology, business |
| الوصف: | Liposomes are widely applied in research, diagnostics, medicine and in industry. In this study we show for the first time the effect of hydrodynamic cavitation on liposome stability and compare it to the effect of well described chemical, physical and mechanical treatments. Fluorescein loaded giant 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipid vesicles were treated with hydrodynamic cavitation as promising method in inactivation of biological samples. Hydrodynamic treatment was compared to various chemical, physical and mechanical stressors such as ionic strength and osmolarity agents (glucose, Na+, Ca2+, and Fe3+), free radicals, shear stresses (pipetting, vortex mixing, rotational shear stress), high pressure, electroporation, centrifugation, surface active agents (Triton X-100, ethanol), microwave irradiation, heating, freezing-thawing, ultrasound (ultrasonic bath, sonotrode). The fluorescence intensity of individual fluorescein loaded lipid vesicles was measured with confocal laser microscopy. The distribution of lipid vesicle size, vesicle fluorescence intensity, and the number of fluorescein loaded vesicles was determined before and after treatment with different stressors. The different environmental stressors were ranked in order of their relative effect on liposome fluorescein release. Of all tested chemical, physical and mechanical treatments for stability of lipid vesicles, the most detrimental effect on vesicles stability had hydrodynamic cavitation, vortex mixing with glass beads and ultrasound. Here we showed, for the first time that hydrodynamic cavitation was among the most effective physico-chemical treatments in destroying lipid vesicles. This work provides a benchmark for lipid vesicle robustness to a variety of different physico-chemical and mechanical parameters important in lipid vesicle preparation and application. |
| وصف الملف: | application/pdf; application/msword; text/url |
| تدمد: | 1350-4177 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c2e732a3cf505f957d61254f0c6f562 https://doi.org/10.1016/j.ultsonch.2019.104826 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8c2e732a3cf505f957d61254f0c6f562 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13504177 |
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